Document Detail

A geometric model for 3-D confocal image analysis.
MedLine Citation:
PMID:  11125595     Owner:  NLM     Status:  MEDLINE    
In this paper, we use partial-differential-equation-based filtering as a preprocessing and post processing strategy for computer-aided cytology. We wish to accurately extract and classify the shapes of nuclei from confocal microscopy images, which is a prerequisite to an accurate quantitative intranuclear (genotypic and phenotypic) and internuclear (tissue structure) analysis of tissue and cultured specimens. First, we study the use of a geometry-driven edge-preserving image smoothing mechanism before nuclear segmentation. We show how this filter outperforms other widely-used filters in that it provides higher edge fidelity. Then we apply the same filter, with a different initial condition, to smooth nuclear surfaces and obtain sub-pixel accuracy. Finally we use another instance of the geometrical filter to correct for misinterpretations of the nuclear surface by the segmentation algorithm. Our prefiltering and post filtering nicely complements our initial segmentation strategy, in that it provides substantial and measurable improvement in the definition of the nuclear surfaces.
A Sarti; C Ortiz de Solórzano; S Lockett; R Malladi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Validation Studies    
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  47     ISSN:  0018-9294     ISO Abbreviation:  IEEE Trans Biomed Eng     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2001-02-01     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1600-9     Citation Subset:  IM    
Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Bias (Epidemiology)
Cell Nucleus / ultrastructure*
Computer Simulation*
Image Interpretation, Computer-Assisted / methods*
Imaging, Three-Dimensional / methods*
Microscopy, Confocal / methods*
Signal Processing, Computer-Assisted*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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