Document Detail


A genome-wide role for CHD remodelling factors and Nap1 in nucleosome disassembly.
MedLine Citation:
PMID:  17510629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromatin remodelling factors and histone chaperones were previously shown to cooperatively affect nucleosome assembly and disassembly processes in vitro. Here, we show that Schizosaccharomyces pombe CHD remodellers, the Hrp1 and Hrp3 paralogs physically interact with the histone chaperone Nap1. Genome-wide analysis of Hrp1, Hrp3 and Nap1 occupancy, combined with nucleosome density measurements revealed that the CHD factors and Nap1 colocalized in particular to promoter regions where they remove nucleosomes near the transcriptional start site. Hrp1 and Hrp3 also regulate nucleosome density in coding regions, where they have redundant roles to stimulate transcription. Previously, DNA replication-dependent and -independent nucleosome disassembly processes have been described. We found that nucleosome density increased in the hrp1 mutant in the absence of DNA replication. Finally, regions where nucleosome density increased in hrp1, hrp3 and nap1 mutants also showed nucleosome density and histone modification changes in HDAC and HAT mutants. Thus, this study revealed an important in vivo role for CHD remodellers and Nap1 in nucleosome disassembly at promoters and coding regions, which are linked to changes in histone acetylation.
Authors:
Julian Walfridsson; Olga Khorosjutina; Paulina Matikainen; Claes M Gustafsson; Karl Ekwall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-17
Journal Detail:
Title:  The EMBO journal     Volume:  26     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-09-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  2868-79     Citation Subset:  IM    
Affiliation:
Karolinska Institutet, Department of Biosciences and Medical Nutrition/School of Life Sciences, University College Sodertorn, Alfred Nobel's Allé 7, 141 89 Huddinge, Sweden. julian.walfridsson@sh.se
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE6557
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Chromatin Assembly and Disassembly*
DNA Replication
Fungal Proteins / physiology*
Gene Expression Regulation, Fungal
Genome, Fungal*
Histones / metabolism
Nucleosomes / metabolism*
Promoter Regions, Genetic
Schizosaccharomyces / genetics*
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Histones; 0/Nucleosomes
Comments/Corrections

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