Document Detail


A genome-wide analysis of courting and mating responses in Drosophila melanogaster females.
MedLine Citation:
PMID:  15499404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Drosophila melanogaster, seminal fluid proteins influence several components of female physiology and behavior, including re-mating rates, ovulation and oviposition, and sperm use. It is well-known that female flies are not simply passive vessels and that female-mediated interactions with male products are important to female (and thus male) reproductive success. While the population genetics, molecular evolution and physiological effects of seminal fluid proteins have been examined, the genetics and evolution of the female side of these post-mating interactions is unexplored in spite of work showing that female genotype and female-by-male genotype interactions are important determinants of sperm competition outcomes. Here we use microarrays to identify candidate genes involved in the female side of post-mating sexual interactions. We report the results of a whole-genome oligonucleotide chip experiment that reveals 23 genes differentially expressed between virgin females exposed and unexposed to courting males, and 38 genes differentially expressed between virgin and recently mated females. Immune related genes are overrepresented among the mating-influenced candidates. We use quantitative reverse-transcriptase PCR to independently assess gene expression changes for roughly half of the mating-affected candidate genes.
Authors:
Mara K N Lawniczak; David J Begun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  47     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-22     Completed Date:  2005-03-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  900-10     Citation Subset:  IM    
Affiliation:
Center for Population Biology, Section of Evolution and Ecology, University of California at Davis, One Shields Avenue, CA 95616, USA. marakat@ucdavis.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila Proteins / metabolism
Drosophila melanogaster / genetics,  physiology
Female
Gene Expression Profiling / methods
Gene Expression Regulation / genetics,  physiology*
Genes, Insect / genetics,  physiology*
Genotype
Male
Oviposition / genetics,  physiology*
Ovulation / genetics,  physiology
Reproduction / genetics,  physiology*
Seminal Plasma Proteins / metabolism
Sexual Behavior, Animal / physiology*
Spermatozoa / metabolism
Grant Support
ID/Acronym/Agency:
GM55298/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Seminal Plasma Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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