| A genome map of Salmonella enterica serovar Agona: numerous insertions and deletions reflecting the evolutionary history of a human pathogen. | |
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MedLine Citation:
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PMID: 19533840 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salmonella enterica serovar Agona is an important zoonotic pathogen, causing serious human illness worldwide, but knowledge about its genetics and evolution, especially regarding the genomic events that might have contributed to the formation of S. Agona as an important pathogen, is lacking. As a first step toward understanding this pathogen and characterizing its genomic differences with other salmonellae, we constructed a physical map of S. Agona in strain SARB1 using I-CeuI, XbaI, AvrII and Tn10 insertions with pulsed-field gel electrophoresis techniques. On the 4815-kb genomic map, we located 82 genes, revealed one inversion of about 1000 kb and resolved seven deletions and seven insertions ranging from 10 to 67 kb relative to the genome of Salmonella typhimurium LT2. These genomic features clearly distinguish S. Agona from other previously analyzed salmonellae and provide clues to the molecular basis for its genomic divergence. Additionally, these kinds of physical maps, combined with emerging high-speed sequencing technologies, such as the Solexa or SOLiD techniques, which require a pre-existing high-resolution physical map such as the S. Agona map reported here, will play important roles in genomic comparative studies of bacteria involving large numbers of strains. |
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Authors:
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Fang Chen; Cornelis Poppe; Gui-Rong Liu; Yong-Guo Li; Yi-Hong Peng; Kenneth E Sanderson; Randal N Johnston; Shu-Lin Liu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology letters Volume: 293 ISSN: 1574-6968 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-06-16 Completed Date: 2009-08-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 188-95 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Peking University Health Science Center, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Inversion DNA Transposable Elements DNA, Bacterial / genetics* Electrophoresis, Gel, Pulsed-Field Evolution, Molecular* Genome, Bacterial* Humans Mutagenesis, Insertional Recombination, Genetic Restriction Mapping* Salmonella enterica / genetics* Salmonella typhimurium / genetics Sequence Deletion |
| Chemical | |
Reg. No./Substance:
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0/DNA Transposable Elements; 0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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