| The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology. | |
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MedLine Citation:
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PMID: 18723656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polaromonas sp. strain JS666 can grow on cis-1,2-dichloroethene (cDCE) as a sole carbon and energy source and may be useful for bioremediation of chlorinated solvent-contaminated sites. Analysis of the genome sequence of JS666 (5.9 Mb) shows a bacterium well adapted to pollution that carries many genes likely to be involved in hydrocarbon and xenobiotic catabolism and metal resistance. Clusters of genes coding for haloalkane, haloalkanoate, n-alkane, alicyclic acid, cyclic alcohol, and aromatic catabolism were analyzed in detail, and growth on acetate, catechol, chloroacetate, cyclohexane carboxylate, cyclohexanol, ferulate, heptane, 3-hydroxybenzoate, hydroxyquinol, gentisate, octane, protocatechuate, and salicylate was confirmed experimentally. Strain JS666 also harbors diverse putative mobile genetic elements, including retrons, inteins, a miniature inverted-repeat transposable element, insertion sequence transposases from 14 families, eight genomic islands, a Mu family bacteriophage, and two large (338- and 360-kb) plasmids. Both plasmids are likely to be self-transferable and carry genes for alkane, alcohol, aromatic, and haloacid metabolism. Overall, the JS666 genome sequence provides insights into the evolution of pollutant-degrading bacteria and provides a toolbox of catabolic genes with utility for biotechnology. |
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Authors:
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Timothy E Mattes; Anne K Alexander; Paul M Richardson; A Christine Munk; Cliff S Han; Paul Stothard; Nicholas V Coleman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-08-22 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 74 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-14 Completed Date: 2008-11-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 6405-16 Citation Subset: IM |
Affiliation:
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Department of Civil and Environmental Engineering, 4105 Seamans Center, The University of Iowa, Iowa City, Iowa 52242, USA. tim-mattes@uiowa.edu |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/CP000316; CP000317; CP000318 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics* Comamonadaceae / genetics* DNA, Bacterial / chemistry, genetics* Enzymes / genetics* Evolution, Molecular* Genes, Bacterial Genome, Bacterial* Hydrocarbons / metabolism Interspersed Repetitive Sequences Molecular Sequence Data Multigene Family Sequence Analysis, DNA Xenobiotics / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/Enzymes; 0/Hydrocarbons; 0/Xenobiotics |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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