Document Detail


A genetically encoded photosensitizer.
MedLine Citation:
PMID:  16369538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase Cdelta1 pleckstrin homology domain.
Authors:
Maria E Bulina; Dmitriy M Chudakov; Olga V Britanova; Yurii G Yanushevich; Dmitry B Staroverov; Tatyana V Chepurnykh; Ekaterina M Merzlyak; Maria A Shkrob; Sergey Lukyanov; Konstantin A Lukyanov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-20
Journal Detail:
Title:  Nature biotechnology     Volume:  24     ISSN:  1087-0156     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-11     Completed Date:  2006-03-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  95-9     Citation Subset:  IM    
Affiliation:
Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY969116
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Line
Cell Proliferation / radiation effects
Cell Survival / radiation effects
Escherichia coli / physiology,  radiation effects*
Green Fluorescent Proteins / genetics*,  metabolism*
Humans
Kidney / cytology*,  radiation effects*
Light
Molecular Sequence Data
Photosensitizing Agents / metabolism*
Protein Engineering
Chemical
Reg. No./Substance:
0/Photosensitizing Agents; 0/killer red protein, Anthomedusae; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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