| A genetically encoded photosensitizer. | |
MedLine Citation:
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PMID: 16369538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase Cdelta1 pleckstrin homology domain. |
Authors:
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Maria E Bulina; Dmitriy M Chudakov; Olga V Britanova; Yurii G Yanushevich; Dmitry B Staroverov; Tatyana V Chepurnykh; Ekaterina M Merzlyak; Maria A Shkrob; Sergey Lukyanov; Konstantin A Lukyanov |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-12-20 |
Journal Detail:
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Title: Nature biotechnology Volume: 24 ISSN: 1087-0156 ISO Abbreviation: Nat. Biotechnol. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-11 Completed Date: 2006-03-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9604648 Medline TA: Nat Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 95-9 Citation Subset: IM |
Affiliation:
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Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AY969116 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cell Line Cell Proliferation / radiation effects Cell Survival / radiation effects Escherichia coli / physiology, radiation effects* Green Fluorescent Proteins / genetics*, metabolism* Humans Kidney / cytology*, radiation effects* Light Molecular Sequence Data Photosensitizing Agents / metabolism* Protein Engineering |
| Chemical | |
Reg. No./Substance:
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0/Photosensitizing Agents; 0/killer red protein, Anthomedusae; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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