| A genetically encoded photocaged amino acid. | |
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MedLine Citation:
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PMID: 15521721 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG. |
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Authors:
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Ning Wu; Alexander Deiters; T Ashton Cropp; David King; Peter G Schultz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 126 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-11-03 Completed Date: 2004-12-16 Revised Date: 2012-07-27 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 14306-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cysteine / analogs & derivatives*, metabolism Histidine / genetics, metabolism Humans Leucine-tRNA Ligase / genetics*, metabolism* Methyltyrosines / genetics, metabolism* Models, Molecular Molecular Sequence Data Mutagenesis Nucleic Acid Conformation Octanoic Acids / metabolism* Photochemistry Proteins / genetics RNA, Transfer, Amino Acyl / genetics, metabolism Superoxide Dismutase / genetics |
| Grant Support | |
ID/Acronym/Agency:
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GM62159/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Methyltyrosines; 0/Octanoic Acids; 0/Proteins; 0/RNA, Transfer, Amino Acyl; 3308-72-3/O-methyltyrosine; 52-90-4/Cysteine; 71-00-1/Histidine; EC 1.15.1.1/Superoxide Dismutase; EC 6.1.1.4/Leucine-tRNA Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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