Document Detail

A genetically encoded photocaged amino acid.
MedLine Citation:
PMID:  15521721     Owner:  NLM     Status:  MEDLINE    
We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.
Ning Wu; Alexander Deiters; T Ashton Cropp; David King; Peter G Schultz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  126     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-03     Completed Date:  2004-12-16     Revised Date:  2012-07-27    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14306-7     Citation Subset:  IM    
Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
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MeSH Terms
Base Sequence
Cysteine / analogs & derivatives*,  metabolism
Histidine / genetics,  metabolism
Leucine-tRNA Ligase / genetics*,  metabolism*
Methyltyrosines / genetics,  metabolism*
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation
Octanoic Acids / metabolism*
Proteins / genetics
RNA, Transfer, Amino Acyl / genetics,  metabolism
Superoxide Dismutase / genetics
Grant Support
Reg. No./Substance:
0/Methyltyrosines; 0/Octanoic Acids; 0/Proteins; 0/RNA, Transfer, Amino Acyl; 3308-72-3/O-methyltyrosine; 52-90-4/Cysteine; 71-00-1/Histidine; EC Dismutase; EC Ligase

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