Document Detail


A genetic strategy to overcome the senescence of primary meningioma cell cultures.
MedLine Citation:
PMID:  16554968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Even though meningiomas are the second most common brain tumor in adults, little is known about the molecular basis of their growth and development. The lack of suitable cell culture model systems is an impediment to this understanding. Most studies on meningiomas rely on primary, early passage cell lines that eventually senesce or a few established cell lines that have been derived from aggressive variants of meningiomas. We have isolated three primary meningioma cell lines that are negative for telomerase activity. We can overcome the senescence of a Grade III derived meningioma cell line by expressing the telomerase catalytic subunit (hTERT), whereas Grade I meningioma cell lines require the expression of the human papillomavirus E6 and E7 oncogenes in conjunction with hTERT. Meningioma cell lines, immortalized in this manner, maintain their pre-transfection morphology and form colonies in vitro. We have confirmed the meningothelial origin of these cell lines by assessing expression of vimentin and desmoplakin, characteristic markers for meningiomas. Additionally, we have karyotyped these cell lines using array CGH and shown that they represent a spectrum of the genetic diversity seen in primary meningiomas. Thus, these cell lines represent novel cellular reagents for investigating the molecular oncogenesis of meningiomas.
Authors:
Gilson S Baia; Alison L Slocum; Jeanette D Hyer; Anjan Misra; Nouzhan Sehati; Scott R VandenBerg; Burt G Feuerstein; Dennis F Deen; Michael W McDermott; Anita Lal
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-23
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  78     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-01     Completed Date:  2006-09-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  113-21     Citation Subset:  IM    
Affiliation:
Brain Tumor Research Center, Department of Neurological Surgery, University of California, San Francisco, CA 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / genetics*,  physiology
Cell Culture Techniques / methods
Cell Line, Tumor / metabolism*
DNA-Binding Proteins / genetics*,  metabolism
Desmoplakins / metabolism
Gene Expression Regulation, Neoplastic / genetics,  physiology*
Gene Transfer Techniques
Humans
Karyotyping
Meningeal Neoplasms / genetics*,  metabolism
Meningioma / genetics*,  metabolism
Oncogene Proteins, Viral / metabolism*
Papillomaviridae
Papillomavirus E7 Proteins / metabolism
Telomerase / genetics*,  metabolism
Transfection / methods
Transformation, Genetic / genetics*
Tumor Markers, Biological / metabolism*
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Desmoplakins; 0/E6 protein, Human papillomavirus type 18; 0/Oncogene Proteins, Viral; 0/Papillomavirus E7 Proteins; 0/Tumor Markers, Biological; 0/Vimentin; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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