Document Detail


A genetic method for generating Drosophila eyes composed exclusively of mitotic clones of a single genotype.
MedLine Citation:
PMID:  10430588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The genetic analysis of a gene at a late developmental stage can be impeded if the gene is required at an earlier developmental stage. The construction of mosaic animals, particularly in Drosophila, has been a means to overcome this obstacle. However, the phenotypic analysis of mitotic clones is often complicated because standard methods for generating mitotic clones render mosaic tissues that are a composite of both mutant and phenotypically normal cells. We describe here a genetic method (called EGUF/hid) that uses both the GAL4/UAS and FLP/FRT systems to overcome this limitation for the Drosophila eye by producing genetically mosaic flies that are otherwise heterozygous but in which the eye is composed exclusively of cells homozygous for one of the five major chromosome arms. These eyes are nearly wild type in size, morphology, and physiology. Applications of this genetic method include phenotypic analysis of existing mutations and F(1) genetic screens to identify as yet unknown genes involved in the biology of the fly eye. We illustrate the utility of the method by applying it to lethal mutations in the synaptic transmission genes synaptotagmin and syntaxin.
Authors:
R S Stowers; T L Schwarz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  152     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-10-12     Completed Date:  1999-10-12     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1631-9     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium-Binding Proteins*
Cell Lineage
Chromosomes / genetics,  ultrastructure
Clone Cells
Crosses, Genetic
Drosophila melanogaster / genetics*
Electroretinography
Eye / cytology*
Genes, Lethal
Genetic Techniques*
Genotype
Insect Proteins / genetics,  physiology
Membrane Glycoproteins / genetics,  physiology
Membrane Proteins / genetics,  physiology
Microscopy, Electron, Scanning
Mosaicism
Nerve Tissue Proteins / genetics,  physiology
Qa-SNARE Proteins
Recombination, Genetic
Synaptotagmins
Grant Support
ID/Acronym/Agency:
1 F32 NS10561-01/NS/NINDS NIH HHS; MH 48108/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Insect Proteins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Qa-SNARE Proteins; 134193-27-4/Synaptotagmins
Comments/Corrections

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