Document Detail


A genetic deficiency that spans the flightin gene of Drosophila melanogaster affects the ultrastructure and function of the flight muscles.
MedLine Citation:
PMID:  9622575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a reverse-genetic approach to study the function of flightin, a unique protein of the flight muscle myofibril of Drosophila melanogaster. We describe the generation and characterization of Df(3L)fln1, a lethal genetic deficiency in the 76BE region of the third chromosome which deletes several genes, including the gene for flightin. We show that heterozygous flies harboring the Df(3L)fln1 mutation exhibit both impaired flight and ultrastructural defects in their flight muscle myofibrils. We found that the mutation does not interfere with assembly of the myofibril but leads to disorganization of peripheral myofilaments in adult myofibrils. Most myofibrils, nevertheless, retain an intact core that represents approximately 80 % of the normal lattice diameter. Mechanical analysis of single skinned flight muscle fibers demonstrates that the mutation has no significant effect on net power output but increases the frequency at which maximum power is delivered to the wings, potentially reducing the overall performance of the flight system. The results suggest that flightin is an indispensable part of the flight muscle contractile mechanism.
Authors:
J O Vigoreaux; C Hernandez; J Moore; G Ayer; D Maughan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  201     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-08-06     Completed Date:  1998-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2033-44     Citation Subset:  IM    
Affiliation:
Department of Biology and Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA. jvigorea@zoo.uvm.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Drosophila Proteins
Drosophila melanogaster / genetics*,  ultrastructure
Flight, Animal
Gene Deletion*
Kinetics
Microscopy, Electron
Muscle Contraction / genetics,  physiology
Muscle Proteins / genetics*,  physiology
Muscles / ultrastructure*
Mutation*
Myofibrils / ultrastructure
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Muscle Proteins; 0/fln protein, Drosophila; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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