Document Detail

The genes for Panton Valentine leukocidin (PVL) are conserved in diverse lines of methicillin-resistant and methicillin-susceptible Staphylococcus aureus.
MedLine Citation:
PMID:  18656408     Owner:  NLM     Status:  MEDLINE    
Methicillin-resistant Staphylococcus aureus isolated in the community (CA-MRSA) have been reported to carry the loci for Panton Valentine leukocidin (PVL) in high frequency. CA-MRSA in Orebro County, Sweden, constitutes at least 50% of MRSA and the PVL locus is detected in as many as 66% of these CA-MRSA isolates. The aim of this study was to characterize PVL-positive methicillin-resistant and methicillin-susceptible Staphylococcus aureus by molecular methods, to determine the nucleotide sequence of lukS-PV and lukF-PV in S. aureus isolates of different origins, and to investigate the biological consequence of variations occurring in the genes. The PVL-positive MRSA investigated were composed of six different STs (ST8, 36, 80, 152, 154, and 256). Six additional STs (ST5, 22, 25, 30, 88, and 567) were detected when investigating PVL-positive methicillin-susceptible S. aureus with MLST. Despite the different genetic origins of the isolates analyzed, the PVL genes were well conserved and only one mutation was non-synonymous. Evaluation of the consequence of this mutation showed that the mutated toxin and wild-type toxin had comparable biological activity on human polymorphonuclear cells.
Carolina Berglund; Gilles Prévost; Benoît-Joseph Laventie; Daniel Keller; Bo Söderquist
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-07
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  10     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2008-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  France    
Other Details:
Languages:  eng     Pagination:  878-84     Citation Subset:  IM    
Department of Clinical Microbiology, Orebro University Hospital, 70185 Orebro, Sweden.
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MeSH Terms
Bacterial Proteins / genetics,  toxicity
Bacterial Toxins / genetics*,  toxicity
Cluster Analysis
Community-Acquired Infections / microbiology
Conserved Sequence*
DNA, Bacterial / chemistry,  genetics
Exotoxins / genetics*,  toxicity
Leukocidins / genetics*,  toxicity
Methicillin Resistance*
Neutrophils / drug effects
Sequence Analysis, DNA
Staphylococcal Infections / microbiology
Staphylococcus aureus / classification,  drug effects*,  genetics*,  isolation & purification
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Toxins; 0/DNA, Bacterial; 0/Exotoxins; 0/Leukocidins; 0/Panton-Valentine leukocidin

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