| The genes for Panton Valentine leukocidin (PVL) are conserved in diverse lines of methicillin-resistant and methicillin-susceptible Staphylococcus aureus. | |
MedLine Citation:
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PMID: 18656408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methicillin-resistant Staphylococcus aureus isolated in the community (CA-MRSA) have been reported to carry the loci for Panton Valentine leukocidin (PVL) in high frequency. CA-MRSA in Orebro County, Sweden, constitutes at least 50% of MRSA and the PVL locus is detected in as many as 66% of these CA-MRSA isolates. The aim of this study was to characterize PVL-positive methicillin-resistant and methicillin-susceptible Staphylococcus aureus by molecular methods, to determine the nucleotide sequence of lukS-PV and lukF-PV in S. aureus isolates of different origins, and to investigate the biological consequence of variations occurring in the genes. The PVL-positive MRSA investigated were composed of six different STs (ST8, 36, 80, 152, 154, and 256). Six additional STs (ST5, 22, 25, 30, 88, and 567) were detected when investigating PVL-positive methicillin-susceptible S. aureus with MLST. Despite the different genetic origins of the isolates analyzed, the PVL genes were well conserved and only one mutation was non-synonymous. Evaluation of the consequence of this mutation showed that the mutated toxin and wild-type toxin had comparable biological activity on human polymorphonuclear cells. |
Authors:
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Carolina Berglund; Gilles Prévost; Benoît-Joseph Laventie; Daniel Keller; Bo Söderquist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-07 |
Journal Detail:
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Title: Microbes and infection / Institut Pasteur Volume: 10 ISSN: 1286-4579 ISO Abbreviation: Microbes Infect. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-08-11 Completed Date: 2008-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883508 Medline TA: Microbes Infect Country: France |
Other Details:
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Languages: eng Pagination: 878-84 Citation Subset: IM |
Affiliation:
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Department of Clinical Microbiology, Orebro University Hospital, 70185 Orebro, Sweden. carolina.berglund@orebroll.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
toxicity Bacterial Toxins / genetics*, toxicity Cluster Analysis Community-Acquired Infections / microbiology Conserved Sequence* DNA, Bacterial / chemistry, genetics Exotoxins / genetics*, toxicity Genotype Humans Leukocidins / genetics*, toxicity Methicillin Resistance* Neutrophils / drug effects Sequence Analysis, DNA Staphylococcal Infections / microbiology Staphylococcus aureus / classification, drug effects*, genetics*, isolation & purification Sweden |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Bacterial Toxins; 0/DNA, Bacterial; 0/Exotoxins; 0/Leukocidins; 0/Panton-Valentine leukocidin |
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