| The generation of culture activated killer cells (AK) is interleukin-2-dependent and requires self-Ia recognition. | |
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MedLine Citation:
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PMID: 3921469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytotoxic cells which lack major histocompatibility restriction for lysis are generated when murine spleen cells are cultured in the presence of FCS with or without allogeneic stimulation. The studies reported show that, following 3 days of culture with FCS, murine spleen cell cultures contain at least two cytotoxic populations. The first (AK-YAC), expresses an NK cell phenotype and target specificity while the second (AK-WEHI) shares the characteristics of the NC cell. Generation of the AK-YAC effector cell requires the presence of a pre-AK cytotoxic cell (Qa-5+, Lyt-2-) and is dependent on the generation of interleukin-2 (IL-2) during the culture period, while the AK-WEHI effector is independent of IL-2 production. IL-2 production in the cultures is shown to require syngeneic Ia recognition by an Lyt-1+, L3T4+ T cell. These findings suggest a role for IL-2 in the in vivo regulation of NCMC and describe a mechanism for its production in the absence of antigenic stimulation. |
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Authors:
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E C Lattime; O Stutman |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 35 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1985 Apr |
Date Detail:
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Created Date: 1985-06-05 Completed Date: 1985-06-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: DENMARK |
Other Details:
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Languages: eng Pagination: 535-42 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Cell Survival Cells, Cultured Histocompatibility Antigens Class II / immunology Interleukin-2 / analysis, immunology* Killer Cells, Natural / immunology* Lymphocyte Activation Lymphocytes / immunology* Lymphoma Mast-Cell Sarcoma Mice Mice, Inbred C57BL Sarcoma Spleen / immunology T-Lymphocytes, Cytotoxic / immunology |
| Grant Support | |
ID/Acronym/Agency:
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CA-08748/CA/NCI NIH HHS; CA-15988/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Histocompatibility Antigens Class II; 0/Interleukin-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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