Document Detail


A gene regulatory network controlling the embryonic specification of endoderm.
MedLine Citation:
PMID:  21623371     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Specification of endoderm is the prerequisite for gut formation in the embryogenesis of bilaterian organisms. Modern lineage labelling studies have shown that in the sea urchin embryo model system, descendants of the veg1 and veg2 cell lineages produce the endoderm, and that the veg2 lineage also gives rise to mesodermal cell types. It is known that Wnt/β-catenin signalling is required for endoderm specification and Delta/Notch signalling is required for mesoderm specification. Some direct cis-regulatory targets of these signals have been found and various phenomenological patterns of gene expression have been observed in the pre-gastrular endomesoderm. However, no comprehensive, causal explanation of endoderm specification has been conceived for sea urchins, nor for any other deuterostome. Here we propose a model, on the basis of the underlying genomic control system, that provides such an explanation, built at several levels of biological organization. The hardwired core of the control system consists of the cis-regulatory apparatus of endodermal regulatory genes, which determine the relationship between the inputs to which these genes are exposed and their outputs. The architecture of the network circuitry controlling the dynamic process of endoderm specification then explains, at the system level, a sequence of developmental logic operations, which generate the biological process. The control system initiates non-interacting endodermal and mesodermal gene regulatory networks in veg2-derived cells and extinguishes the endodermal gene regulatory network in mesodermal precursors. It also generates a cross-regulatory network that specifies future anterior endoderm in veg2 descendants and institutes a distinct network specifying posterior endoderm in veg1-derived cells. The network model provides an explanatory framework that relates endoderm specification to the genomic regulatory code.
Authors:
Isabelle S Peter; Eric H Davidson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-05-29
Journal Detail:
Title:  Nature     Volume:  474     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-07-01     Completed Date:  2011-07-11     Revised Date:  2014-04-07    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  635-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning
Endoderm / embryology*
Gastrulation
Gene Expression Regulation, Developmental
Gene Regulatory Networks / genetics*
Mesoderm / embryology
Strongylocentrotus purpuratus / embryology*,  genetics*
Grant Support
ID/Acronym/Agency:
HD37105/HD/NICHD NIH HHS; P01 HD037105/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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