| pRb2/p130 gene overexpression induces astrocyte differentiation. | |
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MedLine Citation:
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PMID: 11273639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are many data on the activity of the RB gene in neural differentiation and apoptosis, but the role of pRb2/p130 in neuronal and glial maturation has been far less investigated. To elucidate the role of pRb2/p130 in astrocyte development we overexpressed this protein in astrocytoma and normal astrocyte cultures by adenoviral-mediated gene transfer. In astrocytoma cells, p130/RB2 overexpression resulted in a significant reduction of cell growth and in an increased G(0)/G(1) cell population. We did not observe any induction of programmed cell death as determined by TUNEL reaction. Interestingly, pRb2/p130 overexpression induced astrocyte differentiation. Astrocyte cell cycle arrest and differentiation seemed to proceed through a way distinct from the p53 pathway. |
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Authors:
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U Galderisi; M A Melone; F P Jori; E Piegari; G Di Bernardo; M Cipollaro; A Cascino; G Peluso; P P Claudio; A Giordano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular neurosciences Volume: 17 ISSN: 1044-7431 ISO Abbreviation: Mol. Cell. Neurosci. Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-29 Completed Date: 2001-06-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9100095 Medline TA: Mol Cell Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 415-25 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Experimental Medicine, Section of Pharmacology, CRISCEB, Second University of Naples, Naples, Italy. umberto.galderisi@unina2.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Apoptosis / physiology Astrocytes / chemistry, cytology*, physiology* Astrocytoma Cell Cycle Proteins* Cell Differentiation / physiology Cell Division / physiology Cell Size / physiology Cyclin-Dependent Kinase Inhibitor p27 Gene Expression / physiology Gene Transfer Techniques Glial Fibrillary Acidic Protein / analysis In Situ Nick-End Labeling Microtubule-Associated Proteins / metabolism Phosphoproteins / genetics* Phosphopyruvate Hydratase / analysis Proteins* RNA, Messenger / analysis Rats Retinoblastoma Protein / genetics* Retinoblastoma-Like Protein p130 Tumor Cells, Cultured Tumor Suppressor Protein p53 / metabolism Tumor Suppressor Proteins* Vimentin / analysis |
| Grant Support | |
ID/Acronym/Agency:
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P01 NS 36466/NS/NINDS NIH HHS; R01 CA 60999-01A1/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cdkn1b protein, rat; 0/Cell Cycle Proteins; 0/Glial Fibrillary Acidic Protein; 0/Microtubule-Associated Proteins; 0/Phosphoproteins; 0/Proteins; 0/RNA, Messenger; 0/Rbl2 protein, rat; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p130; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 0/Vimentin; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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