Document Detail

pRb2/p130 gene overexpression induces astrocyte differentiation.
MedLine Citation:
PMID:  11273639     Owner:  NLM     Status:  MEDLINE    
There are many data on the activity of the RB gene in neural differentiation and apoptosis, but the role of pRb2/p130 in neuronal and glial maturation has been far less investigated. To elucidate the role of pRb2/p130 in astrocyte development we overexpressed this protein in astrocytoma and normal astrocyte cultures by adenoviral-mediated gene transfer. In astrocytoma cells, p130/RB2 overexpression resulted in a significant reduction of cell growth and in an increased G(0)/G(1) cell population. We did not observe any induction of programmed cell death as determined by TUNEL reaction. Interestingly, pRb2/p130 overexpression induced astrocyte differentiation. Astrocyte cell cycle arrest and differentiation seemed to proceed through a way distinct from the p53 pathway.
U Galderisi; M A Melone; F P Jori; E Piegari; G Di Bernardo; M Cipollaro; A Cascino; G Peluso; P P Claudio; A Giordano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular neurosciences     Volume:  17     ISSN:  1044-7431     ISO Abbreviation:  Mol. Cell. Neurosci.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-06-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100095     Medline TA:  Mol Cell Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  415-25     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Experimental Medicine, Section of Pharmacology, CRISCEB, Second University of Naples, Naples, Italy.
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MeSH Terms
Adenoviridae / genetics
Apoptosis / physiology
Astrocytes / chemistry,  cytology*,  physiology*
Cell Cycle Proteins*
Cell Differentiation / physiology
Cell Division / physiology
Cell Size / physiology
Cyclin-Dependent Kinase Inhibitor p27
Gene Expression / physiology
Gene Transfer Techniques
Glial Fibrillary Acidic Protein / analysis
In Situ Nick-End Labeling
Microtubule-Associated Proteins / metabolism
Phosphoproteins / genetics*
Phosphopyruvate Hydratase / analysis
RNA, Messenger / analysis
Retinoblastoma Protein / genetics*
Retinoblastoma-Like Protein p130
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / metabolism
Tumor Suppressor Proteins*
Vimentin / analysis
Grant Support
P01 NS 36466/NS/NINDS NIH HHS; R01 CA 60999-01A1/CA/NCI NIH HHS
Reg. No./Substance:
0/Cdkn1b protein, rat; 0/Cell Cycle Proteins; 0/Glial Fibrillary Acidic Protein; 0/Microtubule-Associated Proteins; 0/Phosphoproteins; 0/Proteins; 0/RNA, Messenger; 0/Rbl2 protein, rat; 0/Retinoblastoma Protein; 0/Retinoblastoma-Like Protein p130; 0/Tumor Suppressor Protein p53; 0/Tumor Suppressor Proteins; 0/Vimentin; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC Hydratase

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