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The gene family coding for the light-harvesting polypeptides of Photosystem I of the red alga Galdieria sulphuraria.
MedLine Citation:
PMID:  16228335     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Recently [Marquardt et al. (2000) Gene 255: 257-265], we isolated a gene encoding a polypeptide of the light-harvesting complex of Photosystem I (LHC I) of the red alga Galdieria sulphuraria. By screening a G. sulphuraria cDNA library with a DNA probe coding for the conserved first transmembrane helix of this protein we isolated four additional genes coding for LHC I polypeptides. The deduced preproteins had calculated molecular masses of 24.6-25.6 kDa and isoelectric points of 8.09-9.82. N-terminal sequencing of a LHC I polypeptide isolated by gel electrophoresis allowed us to determine the cleavage site of the transit peptide of one of the deduced polypeptides. The mature protein has a calculated molecular mass of 20.6 kDa and an isoelectric point of 7.76. The genes were amplified from nuclear G. sulphuraria DNA by polymerase chain reaction (PCR) using oligonucleotides annealing in the regions of the start and stop codons as primers. All genomic sequences were 80-300 base pairs longer than the PCR products obtained from the respective cDNA clones, pointing to the existence of 1-5 introns per gene. The G. sulphuraria genes form a homogeneous gene family with overall pairwise amino acid identities of 46.0-56.6%. Homology to two diatom, one cryptophytic and two higher plant light-harvesting polypeptides was lower with pairwise identities of 21.1-34.1%. Only one diatom polypeptide showed a higher degree of identity of up to -39.3%.
Authors:
J Marquardt; B Lutz; S Wans; E Rhiel; W E Krumbein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photosynthesis research     Volume:  68     ISSN:  1573-5079     ISO Abbreviation:  Photosyn. Res.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954728     Medline TA:  Photosynth Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  121-30     Citation Subset:  -    
Affiliation:
ICBM/Geomikrobiologie, Carl von Ossietzky Universität Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26111, Oldenburg, Germany, jqm12@psu.edu.
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