Document Detail


A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.
MedLine Citation:
PMID:  14586460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.
Authors:
Shiaoching Gong; Chen Zheng; Martin L Doughty; Kasia Losos; Nicholas Didkovsky; Uta B Schambra; Norma J Nowak; Alexandra Joyner; Gabrielle Leblanc; Mary E Hatten; Nathaniel Heintz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nature     Volume:  425     ISSN:  1476-4687     ISO Abbreviation:  Nature     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-30     Completed Date:  2003-12-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  England    
Other Details:
Languages:  eng     Pagination:  917-25     Citation Subset:  IM    
Affiliation:
GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / metabolism
Cell Differentiation
Cell Lineage
Cell Movement
Central Nervous System / cytology,  metabolism*
Chromosomes, Artificial, Bacterial / genetics*
Cyclic Nucleotide Phosphodiesterases, Type 1
Gene Expression Profiling* / methods
Gene Expression Regulation, Developmental
Gene Library*
Genes, Reporter / genetics*
Genetic Vectors / genetics
Green Fluorescent Proteins
Homeodomain Proteins / genetics,  metabolism
Luminescent Proteins / analysis,  genetics
Membrane Glycoproteins / genetics,  metabolism
Mice
Mice, Transgenic
Nerve Tissue Proteins / genetics,  metabolism
Neurosciences / methods
Phosphoric Diester Hydrolases / genetics,  metabolism
Synapses / metabolism
Transgenes / genetics*
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/LHX6 protein, mouse; 0/Luminescent Proteins; 0/Membrane Glycoproteins; 0/Nerve Tissue Proteins; 147336-22-9/Green Fluorescent Proteins; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.17/Cyclic Nucleotide Phosphodiesterases, Type 1
Comments/Corrections
Comment In:
Nature. 2003 Oct 30;425(6961):907-8   [PMID:  14586449 ]

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