Document Detail


A gel-free 3D microfluidic cell culture system.
MedLine Citation:
PMID:  18455231     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
3D microfluidic cell culture systems offer a biologically relevant model to conduct micro-scale mammalian cell-based research and applications. Various natural and synthetic hydrogels have been successfully incorporated into microfluidic systems to support mammalian cells in 3D. However, embedment of cells in hydrogels introduces operational complexity, potentially hinders mass transfer, and is not suitable for establishing cell-dense, ECM-poor constructs. We present here a gel-free method for seeding and culturing mammalian cells three-dimensionally in a microfluidic channel. A combination of transient inter-cellular polymeric linker and micro-fabricated pillar arrays was used for the in situ formation and immobilization of 3D multi-cellular aggregates in a microfluidic channel. 3D cellular constructs formed this way are relieved of hydrogel embedment for cellular support. Two mammalian cell lines (A549 and C3A) and a primary mammalian cell (bone marrow mesenchymal stem cells) were cultured in the gel-free 3D microfluidic cell culture system. The cells displayed 3D cellular morphology, cellular functions and differentiation capability, affirming the versatility of the system as a 3D cell perfusion culture platform for anchorage-dependent mammalian cells.
Authors:
Siew-Min Ong; Chi Zhang; Yi-Chin Toh; So Hyun Kim; Hsien Loong Foo; Choon Hong Tan; Danny van Noort; Sungsu Park; Hanry Yu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-02
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3237-44     Citation Subset:  IM    
Affiliation:
Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Bone Marrow Cells / cytology,  metabolism,  ultrastructure
Cell Aggregation
Cell Culture Techniques / instrumentation,  methods*
Cell Line
Cell Survival
Cells, Cultured
Humans
Hydrogel / chemistry*
Mesenchymal Stem Cells / cytology,  metabolism,  ultrastructure
Microfluidics / instrumentation,  methods*
Microscopy, Electron, Scanning
Models, Biological
Rats
Chemical
Reg. No./Substance:
0/Actins; 25852-47-5/Hydrogel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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