Document Detail


The gas7 protein potentiates NGF-mediated differentiation of PC12 cells.
MedLine Citation:
PMID:  15725398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth-arrest-specific protein gas7 is required for morphological differentiation of cultured mouse cerebellar neurons and PC12 cells. Moreover, its overexpression in various cell types induces neurite-like outgrowth. The role of gas7 in neuronal differentiation was further characterized by adenovirus-mediated overexpression in PC12 cells and quantification of the expression of various neuronal markers, in the absence and presence of different concentrations of nerve growth factor (NGF). The potential neuroprotective activity of gas7 against various neurotoxic insults was also assessed. In addition to promoting the formation of neurite-like extensions, overexpression of gas7 potentiated NGF-mediated neuronal differentiation of PC12 cells, as shown by the enhanced expression of the neuronal proteins betaIII-tubulin, synaptotagmin, alpha7 subunit of the acetylcholine receptor, and dihydropyrimidinase related protein-3. This effect was exerted independently of cell cycle progression, as gas7 did not affect proliferation of PC12 cells. While some differentiation enhancers protect PC12 cells against lethal insults, gas7 overexpression in PC12 cells did not protect against oxygen-glucose deprivation, the calcium ionophore A23187, or the nitric oxide donor sodium nitroprusside, suggesting that gas7 is not neuroprotective. The ability of gas7 to potentiate neuronal differentiation makes it a potential therapeutic target to promote re-establishment of neuronal connections in the injured or diseased brain, such as following stroke.
Authors:
Karine Lortie; Deqi Huang; Balu Chakravarthy; Tanya Comas; Sheng T Hou; Sue Lin-Chao; Paul Morley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  1036     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-23     Completed Date:  2005-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-34     Citation Subset:  IM    
Affiliation:
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Calcium / metabolism
Cell Differentiation / drug effects,  physiology*
Cell Hypoxia / physiology
Genetic Vectors
Ionophores / pharmacology
Mice
Nerve Growth Factor / pharmacology*
Nerve Tissue Proteins / genetics,  metabolism*
Neurons / cytology,  drug effects,  metabolism*
Neuroprotective Agents / metabolism
Neurotoxins / antagonists & inhibitors,  metabolism
Nitric Oxide Donors / pharmacology
PC12 Cells
Rats
Stem Cells / cytology,  drug effects,  metabolism*
Transfection
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Gas7 protein, mouse; 0/Ionophores; 0/Nerve Tissue Proteins; 0/Neuroprotective Agents; 0/Neurotoxins; 0/Nitric Oxide Donors; 7440-70-2/Calcium; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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