| Gamma-polyglutamic acid (gamma-PGA) produced by Bacillus amyloliquefaciens C06 promoting its colonization on fruit surface. | |
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MedLine Citation:
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PMID: 20638145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacillus amyloliquefaciens C06, an effective biological agent in controlling brown rot of stone fruit caused by Monilinia fructicola, was also found to produce extra-cellular mucilage and form mucoid colonies on semi-solid surfaces. This study aimed to characterize the extra-cellular mucilage produced by B. amyloliquefaciens C06 using transposon mutagenesis and biochemical and physical analyses. The mucilage production in B. amyloliquefaciens C06 was demonstrated to be associated with ywsC gene expression and characterized to be of high molecular weight, consisted of only glutamic acid and linked with non-peptide bonds, thus identified as gamma-polyglutamic acid (gamma-PGA). Compared with wild type B. amyloliquefaciens C06, its mutants deficient in producing gamma-PGA, e.g. M106 and C06DeltaywsC showed less efficiency in biofilm formation, surface adhesion and swarming ability. It was also demonstrated that gamma-PGA was not essential for C06 to form colony on semi-solid surfaces, but was able to improve its colony structure. In vivo evaluation showed that disruption of gamma-PGA production in C06DeltaywsC impaired its efficiency of colonizing apple surfaces. |
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Authors:
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Jun Liu; Dan He; Xiu-zhen Li; Shengfeng Gao; Huijun Wu; Wenzhe Liu; Xuewen Gao; Ting Zhou |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-03 |
Journal Detail:
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Title: International journal of food microbiology Volume: 142 ISSN: 1879-3460 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-10 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 190-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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genetics,
physiology* Bacterial Adhesion Bacterial Proteins / genetics, metabolism Biofilms Fruit / microbiology* Gene Expression Regulation, Bacterial Malus / microbiology Mutation Polyglutamic Acid / analogs & derivatives*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/poly(gamma-glutamic acid); 25513-46-6/Polyglutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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