| The future potential of cryopreservation for assisted reproduction. | |
| | |
MedLine Citation:
|
PMID: 19818745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cryopreservation of human gonadal tissue and oocytes has brought about new and exciting research in reproductive medicine, as well as new cryopreservation techniques that are dramatically improving post-thaw viability and freezing damage. The work done on gonadal tissues is aimed at improving the quality of life for infertile patients and for prepubertal patients undergoing gonadotoxic chemotherapy, patients for whom hormonal stimulation/IVF is not an option, and women without partners. Cryopreservation of mature oocytes is the best model for timing IVF. Vitrification is likely to benefit the field, and since 2005, implantation and pregnancy rates from vitrified oocytes have matched or eclipsed results from conventional methods, due to new cell-specific methods and formulas. Cryopreservation of immature oocytes leads to a new direction of egg banking in future. Preserving ovarian tissue for autografting is still promising and has resulted in folliculogenesis, resumed hormone production and live births in limited cases. The use of small cortical blocks, or mechanical/chemical digestion of ovarian tissue for isolation of follicles is a new direction for ovary preservation for reasons of cryoprotectant permeation and graft revascularization. Maturation of follicles in vitro has become more feasible with the use of alginate microencapsulation. Testicular tissue preservation has taken a sharp turn towards preservation of gonadal stem cells. Research into the mechanism for spermatogenesis points to the ability for male germ cells to resume spermatogenesis. The cryopreservation of minced testicular tissue for isolation of germ cells via chemical digestion has produced viable cells, however, structural damage that may be avoided by vitrification has been noted to the surrounding cell junctions and supporting cells. |
| | |
Authors:
|
Ying Song; Randy Sharp; Fenghua Lu; Maliha Hassan |
Related Documents
:
|
19551645 - Hygienic behavior in the stingless bees melipona beecheii and scaptotrigona pectoralis ... 6397475 - Hydrophobic chromatography of cells: adsorption and resolution on homologous series of ... 16510845 - Freeze-thaw increases adeno-associated virus transduction of cells. 16660775 - Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. 19075245 - Paneth cells directly sense gut commensals and maintain homeostasis at the intestinal h... 21725285 - Might makes right: using force to align the mitotic spindle. |
Publication Detail:
|
Type: Journal Article Date: 2009-10-08 |
Journal Detail:
|
Title: Cryobiology Volume: 60 ISSN: 1090-2392 ISO Abbreviation: Cryobiology Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-09-06 Completed Date: 2011-01-20 Revised Date: 2011-08-01 |
Medline Journal Info:
|
Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
|
Languages: eng Pagination: S60-5 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Xytex Research Inc., Augusta, Georgia, USA. yingcsong@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cryopreservation* / methods, trends Cryoprotective Agents* Female Fertilization in Vitro Humans Infertility Insemination, Artificial, Heterologous Insemination, Artificial, Homologous Male Oocytes Organ Preservation / methods Ovarian Follicle Ovary / transplantation Pregnancy Reproductive Techniques, Assisted / trends* Semen Preservation Spermatozoa Testis Tissue Preservation / methods Vitrification |
| Grant Support | |
ID/Acronym/Agency:
|
R43 HD047060-01/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cryoprotective Agents |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Drastic decrease in dopamine receptor levels in the striatum of acetylcholinesterase knock-out mouse...
Next Document: FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish...