Document Detail

The future potential of cryopreservation for assisted reproduction.
MedLine Citation:
PMID:  19818745     Owner:  NLM     Status:  MEDLINE    
Cryopreservation of human gonadal tissue and oocytes has brought about new and exciting research in reproductive medicine, as well as new cryopreservation techniques that are dramatically improving post-thaw viability and freezing damage. The work done on gonadal tissues is aimed at improving the quality of life for infertile patients and for prepubertal patients undergoing gonadotoxic chemotherapy, patients for whom hormonal stimulation/IVF is not an option, and women without partners. Cryopreservation of mature oocytes is the best model for timing IVF. Vitrification is likely to benefit the field, and since 2005, implantation and pregnancy rates from vitrified oocytes have matched or eclipsed results from conventional methods, due to new cell-specific methods and formulas. Cryopreservation of immature oocytes leads to a new direction of egg banking in future. Preserving ovarian tissue for autografting is still promising and has resulted in folliculogenesis, resumed hormone production and live births in limited cases. The use of small cortical blocks, or mechanical/chemical digestion of ovarian tissue for isolation of follicles is a new direction for ovary preservation for reasons of cryoprotectant permeation and graft revascularization. Maturation of follicles in vitro has become more feasible with the use of alginate microencapsulation. Testicular tissue preservation has taken a sharp turn towards preservation of gonadal stem cells. Research into the mechanism for spermatogenesis points to the ability for male germ cells to resume spermatogenesis. The cryopreservation of minced testicular tissue for isolation of germ cells via chemical digestion has produced viable cells, however, structural damage that may be avoided by vitrification has been noted to the surrounding cell junctions and supporting cells.
Ying Song; Randy Sharp; Fenghua Lu; Maliha Hassan
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Publication Detail:
Type:  Journal Article     Date:  2009-10-08
Journal Detail:
Title:  Cryobiology     Volume:  60     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2011-01-20     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S60-5     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Xytex Research Inc., Augusta, Georgia, USA.
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MeSH Terms
Cryopreservation* / methods,  trends
Cryoprotective Agents*
Fertilization in Vitro
Insemination, Artificial, Heterologous
Insemination, Artificial, Homologous
Organ Preservation / methods
Ovarian Follicle
Ovary / transplantation
Reproductive Techniques, Assisted / trends*
Semen Preservation
Tissue Preservation / methods
Grant Support
R43 HD047060-01/HD/NICHD NIH HHS
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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