Document Detail


The future is now: single-cell genomics of bacteria and archaea.
MedLine Citation:
PMID:  23298390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology.
Authors:
Paul C Blainey
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2013-02-11
Journal Detail:
Title:  FEMS microbiology reviews     Volume:  37     ISSN:  1574-6976     ISO Abbreviation:  FEMS Microbiol. Rev.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-08     Completed Date:  2013-09-19     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  8902526     Medline TA:  FEMS Microbiol Rev     Country:  England    
Other Details:
Languages:  eng     Pagination:  407-27     Citation Subset:  IM    
Copyright Information:
© 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Archaea / genetics*
Bacteria / genetics*
Computational Biology
Genetics, Microbial / methods
Genome, Archaeal*
Genome, Bacterial*
Genomics / methods*
High-Throughput Nucleotide Sequencing
Grant Support
ID/Acronym/Agency:
R01 HG004863/HG/NHGRI NIH HHS; R01 HG004863/HG/NHGRI NIH HHS
Comments/Corrections

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