| PML/RARalpha, a fusion protein in acute promyelocytic leukemia, prevents growth factor withdrawal-induced apoptosis in TF-1 cells. | |
| | |
MedLine Citation:
|
PMID: 9816019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A unique mRNA produced by the t(15;17) (q22-24;q11-21) translocation in the leukemic cells of acute promyelocytic leukemia patients encodes a chimeric protein, PML/RARalpha, which is formed by the fusion of the retinoic acid receptor alpha (RARalpha) and the promyelocytic locus gene (PML). This translocation is often the only visible karyotypic aberration present which is detected in almost 100% of acute promyelocytic leukemia patients. As an initial step to study the role of PML/RARalpha in leukemogenesis, we attempted to express the fusion protein in hematopoietic cells through retrovirus-mediated gene transfer of the retroviral vector, pGPRCHT, which contains the PML/RARalpha cDNA. Transduction of the PML/RARalpha cDNA fragment used in this vector, which extends from the position 31 bp to the position 2638 bp in a transcription unit driven by the Moloney murine sarcoma virus LTR, was found to abrogate the growth factor dependence of TF-1 cells. In addition, introduction of PML/RARalpha into TF-1 cells can protect these cells from the apoptosis usually induced in TF-1 cells by growth factor withdrawal, as measured by three assays for apoptosis: morphology, DNA ladder formation, and end labeling of nicked DNA with fluorescent-conjugated nucleotide precursors followed by a fluorescence-activated cell sorting assay. These data suggest that the PML/RARalpha fusion protein may inhibit programmed cell death in myeloid cells. |
| | |
Authors:
|
S Fu; U Consoli; E G Hanania; Z Zu; D F Claxton; M Andreeff; A B Deisseroth |
Related Documents
:
|
18636329 - Over-expression of alpha-actinin with a gfp fusion protein is sufficient to increase wh... 9708829 - A new method to generate quadromas by electrofusion and facs sorting. 1527489 - Ploidy of bacillus subtilis exfusants: the haploid nature of cells forming colonies wit... 16934339 - Discriminating in vitro cell fusion from cell aggregation by flow cytometry combined wi... 6985109 - Effect of water soluble polymer, polyethyleneglycol, and glass-forming compounds on cel... 16945399 - Development and characterization of a rift valley fever virus cell-cell fusion assay us... 9242269 - Anticipatory time intervals of head-direction cells in the anterior thalamus of the rat... 1654769 - Characterization of the vitamin d receptor from the caco-2 human colon carcinoma cell l... 18695639 - Cgmp-independent anti-tumour actions of the inhibitor of soluble guanylyl cyclase, odq,... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Clinical cancer research : an official journal of the American Association for Cancer Research Volume: 1 ISSN: 1078-0432 ISO Abbreviation: Clin. Cancer Res. Publication Date: 1995 Jun |
Date Detail:
|
Created Date: 1999-02-08 Completed Date: 1999-02-08 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9502500 Medline TA: Clin Cancer Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 583-90 Citation Subset: IM |
Affiliation:
|
Department of Hematology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
physiology* Cell Division / drug effects Chromosome Mapping Chromosomes, Human, Pair 15 Chromosomes, Human, Pair 17 Genetic Vectors Growth Substances / pharmacology, physiology Humans Leukemia, Promyelocytic, Acute / genetics* Models, Biological Neoplasm Proteins / genetics*, metabolism* Oncogene Proteins, Fusion / genetics*, metabolism* Recombinant Fusion Proteins / metabolism Retroviridae Reverse Transcriptase Polymerase Chain Reaction Transfection Translocation, Genetic Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
CA16672/CA/NCI NIH HHS; CA57639/CA/NCI NIH HHS; P01 CA55164/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Growth Substances; 0/Neoplasm Proteins; 0/Oncogene Proteins, Fusion; 0/Recombinant Fusion Proteins; 0/promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of the DF3-P epitope in human ovarian carcinomas.
Next Document: Radioimmunoscintigraphy and biodistribution of technetium-99m-labeled monoclonal antibody U36 in pat...