Document Detail


The fungicide propiconazole interferes with embryonic development of the crustacean Daphnia magna.
MedLine Citation:
PMID:  11349850     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Propiconazole is a fungicide used in a variety of agricultural applications. Preliminary studies had suggested that embryos of the crustacean Daphnia magna are particularly susceptible to the toxicity of this chemical. The goals of the present study were to define endpoints of daphnid embryonic development that could be routinely used to assess the embryo toxicity of chemicals and to characterize definitively the embryo toxicity of propiconazole to daphnids. Daphnid embryonic development was characterized into six readily distinguishable stages based on the degree of tissue differentiation. Embryonic development could be monitored either in the brood chamber of the maternal organism or using embryos removed from the brood chamber and incubated ex vivo. Standard toxicity assessment revealed that propiconazole elicited no significant adverse effects on daphnid survival or fecundity during a 21-d exposure to concentrations as high as 0.25 mg/L. Exposure to 0.25 mg/L propiconazole, however, caused a significant incidence of developmental abnormalities and embryonic death. Abnormalities were consistent with developmental arrest at later stages of embryonic maturation. Propiconazole elicited a steep concentration-response curve with respect to embryo toxicity, with a 10% and a 90% incidence of embryo toxicity measured at 0.50 and 0.82 mg/L, respectively. Direct exposure of embryos to propiconazole resulted in toxicity, though the incidence and characteristics of developmental abnormalities were not consistent with that observed during chronic exposures. However, maternal exposure to propiconazole followed by transfer of early embryos to propiconazole-free media resulted in embryo toxicity consistent with that observed during chronic exposure. These results indicate that propiconazole interferes with the later stages of daphnid embryonic development, and that this toxicity is manifested largely via maternal exposure to the fungicide.
Authors:
K Kast-Hutcheson; C V Rider; G A LeBlanc
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  20     ISSN:  0730-7268     ISO Abbreviation:  Environ. Toxicol. Chem.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-05-14     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  502-9     Citation Subset:  IM    
Affiliation:
Department of Toxicology, North Carolina State University, Raleigh 27695-7633, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Daphnia / drug effects*,  embryology*,  growth & development
Dose-Response Relationship, Drug
Female
Fungicides, Industrial / toxicity*
Triazoles / toxicity*
Chemical
Reg. No./Substance:
0/Fungicides, Industrial; 0/Triazoles; 60207-90-1/propiconazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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