Document Detail

The functional significance of absence: the chromosomal segment harboring Tp53 is absent from the T55 rat radiation hybrid mapping panel.
MedLine Citation:
PMID:  12036299     Owner:  NLM     Status:  MEDLINE    
The T55 rat radiation hybrid (RH) mapping panel has been reported to retain the entire rat genome at retention frequencies between 22% and 37%. However, we found that a small segment of rat chromosome 10 harboring at least four different genes, including Tp53, was completely absent from the panel (retention frequency = 0%). Two other markers located in the vicinity exhibited much reduced retention (2-6%). RH clones are generated by transferring highly fragmented DNA into a recipient cell. There might be a strong selection against the transfer and retention of chromosome segments harboring an intact Tp53, as the action of this gene might prevent proliferation and establishment of the RH clone. Our finding further suggests that unexpected low retention or absence of chromosome segments in an RH panel may represent indications that the segments harbor genes with important functions in cell proliferation control.
Afrouz Behboudi; Leyla Roshani; Lisa Lundin; Fredrik Ståhl; Karin Klinga Levan; Göran Levan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  79     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-30     Completed Date:  2002-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  844-8     Citation Subset:  IM    
Copyright Information:
(c)2002 Elsevier Science (USA).
Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, SE 405 30 Göteborg, Sweden.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF410810;  AF410814;  AF449445;  AF452896
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MeSH Terms
Chromosome Mapping
Gene Deletion*
Genes, p53*
Genetic Markers
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Radiation Hybrid Mapping*
Reg. No./Substance:
0/Genetic Markers

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