Document Detail

The functional significance of absence: the chromosomal segment harboring Tp53 is absent from the T55 rat radiation hybrid mapping panel.
MedLine Citation:
PMID:  12036299     Owner:  NLM     Status:  MEDLINE    
The T55 rat radiation hybrid (RH) mapping panel has been reported to retain the entire rat genome at retention frequencies between 22% and 37%. However, we found that a small segment of rat chromosome 10 harboring at least four different genes, including Tp53, was completely absent from the panel (retention frequency = 0%). Two other markers located in the vicinity exhibited much reduced retention (2-6%). RH clones are generated by transferring highly fragmented DNA into a recipient cell. There might be a strong selection against the transfer and retention of chromosome segments harboring an intact Tp53, as the action of this gene might prevent proliferation and establishment of the RH clone. Our finding further suggests that unexpected low retention or absence of chromosome segments in an RH panel may represent indications that the segments harbor genes with important functions in cell proliferation control.
Afrouz Behboudi; Leyla Roshani; Lisa Lundin; Fredrik Ståhl; Karin Klinga Levan; Göran Levan
Related Documents :
8995759 - A rat mutation producing demyelination (dmy) maps to chromosome 17.
12208139 - The rat shorn mutation (shn) maps between d7got143 and d7rat94.
9870869 - Identification of a new non-major histocompatibility complex genetic locus on chromosom...
12905489 - A comparative genetic analysis between collagen-induced arthritis and pristane-induced ...
4006519 - The thyroglobulin gene resides on chromosome 8 in man and on chromosome 7 in the rat.
19620519 - Dissection of chromosome 18 blood pressure and salt-sensitivity quantitative trait loci...
8774479 - Bronchioloalveolar lung carcinomas: k-ras mutations are constant events in the mucinous...
1976629 - Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 a...
20391329 - Prenatal diagnosis of a partial trisomy 13q (q14-->qter): phenotype, cytogenetics and m...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genomics     Volume:  79     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-30     Completed Date:  2002-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  844-8     Citation Subset:  IM    
Copyright Information:
(c)2002 Elsevier Science (USA).
Department of Cell and Molecular Biology-Genetics, Lundberg Laboratory, Göteborg University, SE 405 30 Göteborg, Sweden.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF410810;  AF410814;  AF449445;  AF452896
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromosome Mapping
Gene Deletion*
Genes, p53*
Genetic Markers
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Radiation Hybrid Mapping*
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Annotation of human chromosome 21 for relevance to Down syndrome: gene structure and expression anal...
Next Document:  Structure and evolution of neurexin genes: insight into the mechanism of alternative splicing.