| A functional gltB gene is essential for utilization of acidic amino acids and expression of periplasmic glutaminase/asparaginase (PGA) by Pseudomonas putida KT2440. | |
| | |
MedLine Citation:
|
PMID: 14624355 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pseudomonas putida KT2440, a root-colonizing fluorescent pseudomonad, is capable of utilizing acidic amino acids (Asp and Glu) and their amides (Asn and Gln) as its sole source of carbon and nitrogen. The uptake of Gln and Asn is facilitated by a periplasmic glutaminase/asparaginase (PGA), which hydrolyses Asn and Gln to the respective dicarboxylates. Here, we describe transposon mutagenesis of P. putida KT2440 with a self-cloning promoter probe vector, Tn 5-OT182. Transconjugants defective in Glu-mediated PGA induction were selected for further studies. In most clones the transposon was found to have integrated into the gltB gene, which encodes the major subunit of the glutamate synthase (GOGAT). The transconjugants were nonmotile, no longer showed a chemotactic response towards amino acids, and could not survive prolonged periods of starvation. The acidic amino acids and their amides supported growth of the transconjugants only when supplied together with glucose, suggesting that the gltB-mutants had lost the ability to utilize amino acids as a carbon source. To confirm that gltB inactivation was the cause of this phenotype, we constructed a mutant with a targeted disruption of gltB. This strain behaved like the clones obtained by random mutagenesis, and failed to express not only PGA but also a number of other Glu-induced proteins. In contrast to wild-type cells, the gltB(-) strain accumulated considerable amounts of both Glu and Gln during long-term incubation. |
| | |
Authors:
|
A M Sonawane; K H Röhm |
Related Documents
:
|
15266055 - Metabolic responses to the reduction in palmitate caused by disruption of the fatb gene... 10467355 - Bacterial death induced by expression of the intracellular portion of human fas. 8227155 - Involvement of the conserved acidic amino acid domain of fgf receptor 1 in ligand-recep... 12477895 - K+ amino acid transporter kaat1 mutant y147f has increased transport activity and alter... 10233185 - The effect of cisapride on duodenal acid exposure in the proximal duodenum in healthy s... 18336355 - Surfactin isoforms from bacillus subtilis hso121: separation and characterization. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-11-18 |
Journal Detail:
|
Title: Molecular genetics and genomics : MGG Volume: 271 ISSN: 1617-4615 ISO Abbreviation: Mol. Genet. Genomics Publication Date: 2004 Feb |
Date Detail:
|
Created Date: 2004-02-17 Completed Date: 2004-04-13 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 101093320 Medline TA: Mol Genet Genomics Country: Germany |
Other Details:
|
Languages: eng Pagination: 33-9 Citation Subset: IM |
Affiliation:
|
Institute of Physiological Chemistry, Philipps University, 35033 Marburg, Germany. avinash@mailer.uni-marburg.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amidohydrolases
/
genetics*,
metabolism* Amino Acids, Acidic / metabolism* Base Sequence Cell Division Chemotaxis DNA, Bacterial / genetics Gene Expression Genes, Bacterial* Movement Mutagenesis, Insertional Phenotype Pseudomonas putida / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids, Acidic; 0/DNA, Bacterial; EC 3.5.-/Amidohydrolases; EC 3.5.1.38/glutamin-(asparagin-)ase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Update on the genetics of migraine.
Next Document: Time interval of measles vaccination in patients with Kawasaki disease treated with additional intra...