| The function and differential sorting of a family of aplysia prohormone processing enzymes. | |
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MedLine Citation:
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PMID: 8161455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have cloned four members of the family of subtilisin-like endoproteases expressed in the bag cell neurons of Aplysia and have demonstrated that two of these enzymes are capable of correctly cleaving the egg-laying hormone precursor. The egg-laying hormone precursor undergoes an ordered series of cleavages, such that different peptides are differentially sorted into distinct secretory vesicles. We have used electron microscopic chemistry to demonstrate that at least one processing enzyme is differentially segregated into a class of secretory vesicles containing the bag cell peptides. The segregation of specific endoproteases, along with specific neuropeptides within a given cell type, may ensure appropriate cleavage and prevent inappropriate cleavage of the polyprotein precursors. |
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Authors:
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J Y Chun; J Korner; T Kreiner; R H Scheller; R Axel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuron Volume: 12 ISSN: 0896-6273 ISO Abbreviation: Neuron Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-05-25 Completed Date: 1994-05-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8809320 Medline TA: Neuron Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 831-44 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/L28766; L28767; L28768; L28769; L34741; L38715 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Aplysia / enzymology* Base Sequence Cloning, Molecular DNA, Complementary / chemistry, genetics Furin Invertebrate Hormones / metabolism* Microscopy, Immunoelectron Molecular Sequence Data Neurons / enzymology Neuropeptides / chemistry, genetics Polymerase Chain Reaction Protein Precursors / metabolism* Sequence Homology Serine Endopeptidases / chemistry, genetics Subtilisins / chemistry, genetics, physiology* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Invertebrate Hormones; 0/Neuropeptides; 0/Protein Precursors; 0/egg-laying hormone, Mollusca; EC 3.4.21.-/PC1A protein, Aplysia californica; EC 3.4.21.-/PC1B protein, Aplysia californica; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/Subtilisins; EC 3.4.21.75/Furin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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