Document Detail


The function and differential sorting of a family of aplysia prohormone processing enzymes.
MedLine Citation:
PMID:  8161455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have cloned four members of the family of subtilisin-like endoproteases expressed in the bag cell neurons of Aplysia and have demonstrated that two of these enzymes are capable of correctly cleaving the egg-laying hormone precursor. The egg-laying hormone precursor undergoes an ordered series of cleavages, such that different peptides are differentially sorted into distinct secretory vesicles. We have used electron microscopic chemistry to demonstrate that at least one processing enzyme is differentially segregated into a class of secretory vesicles containing the bag cell peptides. The segregation of specific endoproteases, along with specific neuropeptides within a given cell type, may ensure appropriate cleavage and prevent inappropriate cleavage of the polyprotein precursors.
Authors:
J Y Chun; J Korner; T Kreiner; R H Scheller; R Axel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuron     Volume:  12     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-05-25     Completed Date:  1994-05-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  831-44     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L28766;  L28767;  L28768;  L28769;  L34741;  L38715
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aplysia / enzymology*
Base Sequence
Cloning, Molecular
DNA, Complementary / chemistry,  genetics
Furin
Invertebrate Hormones / metabolism*
Microscopy, Immunoelectron
Molecular Sequence Data
Neurons / enzymology
Neuropeptides / chemistry,  genetics
Polymerase Chain Reaction
Protein Precursors / metabolism*
Sequence Homology
Serine Endopeptidases / chemistry,  genetics
Subtilisins / chemistry,  genetics,  physiology*
Tissue Distribution
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Invertebrate Hormones; 0/Neuropeptides; 0/Protein Precursors; 0/egg-laying hormone, Mollusca; EC 3.4.21.-/PC1A protein, Aplysia californica; EC 3.4.21.-/PC1B protein, Aplysia californica; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/Subtilisins; EC 3.4.21.75/Furin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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