Document Detail


A fumonisin biosynthetic gene cluster in Fusarium oxysporum strain O-1890 and the genetic basis for B versus C fumonisin production.
MedLine Citation:
PMID:  18375156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most species of Fusarium that produce fumonisin mycotoxins produce predominantly B fumonisins (FBs). However, Fusarium oxysporum strain O-1890 produces predominantly C fumonisins (FCs). In this study, the nucleotide sequence of the fumonisin biosynthetic gene (FUM) cluster in strain O-1890 was determined. The order and orientation of FUM genes were the same as in the previously described clusters in Fusarium verticillioides and Fusarium proliferatum. Coding regions of F. oxysporum and F. verticillioides FUM genes were 88-92% identical, but regions flanking the clusters did not share significant identity. The FUM cluster gene FUM8 encodes an alpha-oxoamine synthase, and fum8 mutants of F. verticillioides do not produce fumonisins. Complementation of a fum8 mutant with the F. verticillioidesFUM8 restored FB production. Complementation with F. oxysporumFUM8 also restored production, but the fumonisins produced were predominantly FCs. These data indicate that different orthologues of FUM8 determine whether Fusarium produces predominantly FBs or FCs.
Authors:
Robert H Proctor; Mark Busman; Jeong-Ah Seo; Yin Won Lee; Ronald D Plattner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-23
Journal Detail:
Title:  Fungal genetics and biology : FG & B     Volume:  45     ISSN:  1096-0937     ISO Abbreviation:  Fungal Genet. Biol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-06-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9607601     Medline TA:  Fungal Genet Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1016-26     Citation Subset:  IM    
Affiliation:
National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604-3999, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EU449979;  EU477239
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
DNA, Fungal / chemistry,  genetics
Fumonisins / chemistry,  classification,  metabolism*
Fungal Proteins / chemistry,  genetics,  metabolism
Fusarium / genetics*,  metabolism
Genes, Fungal*
Genetic Complementation Test
Molecular Sequence Data
Multigene Family*
Mutation
Sequence Alignment
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/Fumonisins; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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