| The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood. | |
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MedLine Citation:
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PMID: 2597105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. An improved rat spleen perfusion is described incorporating a method of defibrination which avoids the use of heparin and enables the spleen to be perfused with rat blood for several hours at a haematocrit of 40% and for 12 h or more at a haematocrit of 20%. 2. Glucose oxidation accounted for 11.6% of the total oxygen consumption but this represented only 8% of total glucose uptake, which was largely converted to lactate and released into the perfusate. However, significant amounts of lactate were oxidized. These results can be explained by the presence of at least two cell populations, one emphasizing the anaerobic oxidation of glucose and the other aerobic metabolism, particularly of lactate. 3. Non-esterified fatty acid and 3-hydroxybutyrate, when available at physiological concentrations, were shown to be major oxidative fuels of the spleen. 4. Chylomicron triacylglycerol was hydrolysed readily and taken up, but not oxidized extensively. |
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Authors:
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M A Mindham; P A Mayes |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 263 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1990-01-19 Completed Date: 1990-01-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 325-32 Citation Subset: IM |
Affiliation:
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Department of Veterinary Basic Sciences, Royal Veterinary College, London, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood* Chylomicrons / metabolism Fatty Acids, Nonesterified / metabolism Glucose / metabolism Ketone Bodies / metabolism Lactates / metabolism Lactic Acid Male Oxygen Consumption Perfusion / instrumentation, methods* Potassium / metabolism Rats Rats, Inbred Strains Spleen / blood supply, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Chylomicrons; 0/Fatty Acids, Nonesterified; 0/Ketone Bodies; 0/Lactates; 50-21-5/Lactic Acid; 50-99-7/Glucose; 7440-09-7/Potassium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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