Document Detail

The freezing point depression of mammalian tissues in relation to the question of osmotic activity of cell fluid.
Jump to Full Text
MedLine Citation:
PMID:  13385447     Owner:  NLM     Status:  OLDMEDLINE    
Abstract/OtherAbstract:
The freezing point depression of freshly excised frozen tissues, pulverized in a hydraulic press or in a mortar, is greater than that of plasma. Even at 0 degrees C. the freezing point depression of such homogenates increases significantly with time. Dilution data indicate that such freezing point data are valid. The presence of intact cells has been shown in smears of tissues pulverized in a mortar, but not in smears of those crushed in a hydraulic press. The osmolarity of various diluent solutions affects the calculated osmotic activity of tissue homogenates presumably because of delayed diffusion between the diluent and cell fluid. With a hypertonic NaCl diluent, spuriously low values of tissue osmotic activity are found from calculations assuming instantaneous mixing between homogenates and diluents. The limitations of data from cryoscopic experiments and from tissue-swelling experiments are discussed in relation to the basic question of whether or not cell fluid is isotonic to extracellular fluid.
Authors:
J W APPELBOOM; W A BRODSKY; W H DENNIS; I DIAMOND; J F MILEY; W S REHM
Related Documents :
19494637 - Cryopreservation for corneal storage.
16673907 - Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification.
15196707 - Oocyte cryopreservation: a biological perspective.
18946557 - Cryopreservation and metabolic profiling analysis of arabidopsis t87 suspension-culture...
9286857 - Diadenosine 5',5'''-p1,p4-tetraphosphate (ap4a) controls the timing of cell division in...
8298507 - Glucocorticoid receptor binding in proliferating and differentiating cells: modulation ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  40     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1956 Nov 
Date Detail:
Created Date:  1957-12-01     Completed Date:  2002-05-01     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  183-99     Citation Subset:  OM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Osmosis*
Permeability*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
Download PDF
Copyright © Copyright, 1956, by The Rockefeller Institute for Medical Research
Received Day: 16 Month: 3 Year: 1956
Print publication date: Day: 20 Month: 11 Year: 1956
Volume: 40 Issue: 2
First Page: 183 Last Page: 199
ID: 2147618
PubMed Id: 13385447

THE FREEZING POINT DEPRESSION OF MAMMALIAN TISSUES IN RELATION TO THE QUESTION OF OSMOTIC ACTIVITY OF CELL FLUID
William A. Brodsky
Johannes W. Appelboom
Warren H. Dennis
Warren S. Rehm
John F. Miley
Israel Diamond
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville


Article Categories:
  • Article


Previous Document:  Studies of fluid exchanges between rat liver slices and simple media.
Next Document:  The responses of the pupil of Gekko gekko to external light stimulus.