Document Detail


The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans.
MedLine Citation:
PMID:  15914538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formins are conserved eukaryotic proteins playing key roles in regulating cell polarity. We have characterized the roles of a formin CaBni1p in the polymorphic fungus Candida albicans. CaBni1p localized persistently to hyphal tips during hyphal growth but to distinct growth sites at different cell cycle stages during yeast growth. Cabni1Delta yeast cells exhibited several morphological defects, such as round and enlarged cells, widened bud necks and a random budding pattern. Although Cabni1Delta cells could still undergo yeast-hypha growth switch, the hyphae were markedly swollen. Cabni1Delta also showed defects in spindle and cytoplasmic microtubule orientation and positioning. Coincidentally, the spindle orientation protein CaKar9p in Cabni1Delta yeast cells appeared as multiple random cortical spots, in contrast to the single spot at the bud tip of many wild-type cells. Interestingly, several defects manifested in Cabni1Delta yeast cells were partially corrected during hyphal growth. We found that the second formin CaBnr1p was recruited to hyphal tips, while it localized only to the bud neck during yeast growth. This behavior of CaBnr1p may play a key role in correcting Cabni1Delta defects during hyphal growth. Cabni1Delta exhibited reduced virulence in mice. These results indicate that the formins play an important role in Candida albicans polarized growth and CaBni1p's function is required for virulence.
Authors:
Chang Run Li; Yan Ming Wang; Xin De Zheng; Hui Yan Liang; Jason Chih Wei Tang; Yue Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-05-24
Journal Detail:
Title:  Journal of cell science     Volume:  118     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-09     Completed Date:  2005-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2637-48     Citation Subset:  IM    
Affiliation:
Molecular and Cell Biology Laboratory, Institute of Molecular and Cell Biology, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Candida albicans / cytology*,  genetics,  growth & development,  pathogenicity
Cell Polarity*
Cell Shape
Fungal Proteins / classification,  genetics,  metabolism*
Gene Deletion
Genes, Fungal / genetics
Hyphae / cytology,  growth & development*,  metabolism,  ultrastructure
Mice
Mice, Inbred BALB C
Microtubules / metabolism
Mitotic Spindle Apparatus / metabolism
Mutation / genetics
Protein Transport
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins / genetics,  metabolism
Survival Rate
Virulence
Chemical
Reg. No./Substance:
0/Actins; 0/Fungal Proteins; 0/Saccharomyces cerevisiae Proteins

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