Document Detail


The fork head transcription factor FKTF-1b from Strongyloides stercoralis restores DAF-16 developmental function to mutant Caenorhabditis elegans.
MedLine Citation:
PMID:  16442538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine whether Strongyloides stercoralis FKTF-1, a transcription factor of the FOXO/FKH family and the likely output of insulin/IGF signal transduction in that parasite, has the same or similar developmental regulatory capabilities as DAF-16, its structural ortholog in Caenorhabditis elegans. To this end, both splice variants of the fktf-1 message were expressed under the control of the daf-16alpha promoter in C. elegans carrying loss of function mutations in both daf-2 (the insulin/IGF receptor kinase) and daf-16. Under well-fed culture conditions the majority (91%) of untransformed daf-2; daf-16 double mutants developed via the continuous reproductive cycle, whereas under the same conditions 100% of daf-2 single mutants formed dauers. Transgenic daf-2; daf-16 individuals expressing fktf-1b showed a reversal of the double mutant phenotype with 75% of the population forming dauers under well-fed conditions. This phenotype was even more pronounced than that of daf-2; daf-16 mutants transformed with a homologous rescuing construct, daf-16alpha::daf-16a (56% dauers under well fed conditions), indicating that S. stercoralis fktf-1b can almost fully rescue loss-of-function mutants in C. elegans daf-16. By contrast, daf-2; daf-16 mutants expressing S. stercoralis fktf-1a, encoding the second splice variant of FKTF-1, showed a predominantly continuous pattern of development identical to that of the parental double mutant stock. This indicates that, unlike FKTF-1b, the S. stercoralis transcription factor FKTF-1a cannot trigger the shift to dauer-specific gene expression in C. elegans.
Authors:
Holman C Massey; Mahendra K Bhopale; Xinshe Li; Michelle Castelletto; James B Lok
Related Documents :
3093278 - Early modifications of gene expression induced in liver by azo-dye diet.
9458358 - Epidermal growth factor receptor and insulin-like growth factor-i receptor expression a...
8297478 - Distribution of insulin-like growth factor-i receptor mrna in rat brain. regulation in ...
9106218 - Temperature-regulated mrna accumulation and stabilization for fatty acid desaturase gen...
22454538 - Alternative splicing networks regulated by signaling in human t cells.
11031238 - Cajal bodies: the first 100 years.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2005-12-15
Journal Detail:
Title:  International journal for parasitology     Volume:  36     ISSN:  0020-7519     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-17     Completed Date:  2006-11-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  347-52     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics,  growth & development
Caenorhabditis elegans Proteins / genetics*
DNA, Helminth / genetics
Forkhead Transcription Factors / genetics*
Gene Expression Regulation, Developmental / genetics
Gene Transfer Techniques
Genes, Helminth / genetics
Helminth Proteins / genetics
Insulin / genetics
Larva / genetics
Mutation
Promoter Regions, Genetic / genetics
Signal Transduction / genetics
Strongyloides stercoralis / genetics*
Transcription Factors / genetics*
Transgenes / genetics
Grant Support
ID/Acronym/Agency:
AI-22662/AI/NIAID NIH HHS; AI-50688/AI/NIAID NIH HHS; R01 AI050668/AI/NIAID NIH HHS; RR02512/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/DNA, Helminth; 0/Forkhead Transcription Factors; 0/Helminth Proteins; 0/Insulin; 0/Transcription Factors; 0/daf-16 protein, C elegans
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of microsatellite and antigen-coding loci for differentiating recrudescing Plasmodium fal...
Next Document:  Transmission and burden and the impact of temperature on two species of vertically transmitted micro...