Document Detail


A food-grade process for isolation and partial purification of bacteriocins of lactic acid bacteria that uses diatomite calcium silicate.
MedLine Citation:
PMID:  8633875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriocins, including nisin, pediocin PO2, brevicin 286, and piscicolin 126, were extracted from fermentation media by adsorption onto Micro-Cel (a food-grade diatomite calcium silicate anticaking agent) and subsequent desorption. The optimal conditions for desorption of piscicolin 126 were determined and applied to other bacteriocins, and the relative purities of the desorbed preparations were compared. Piscicolin was not successfully desorbed from Micro-Cel at pH 1.0 to 12.0, with organic solvents, or by increase of ionic strength up to 1 M NaCl. However, 25 and 75% of the bacteriocin activity was desorbed by using 1% sodium deoxycholate and 1% sodium dodecyl sulfate (SDS), respectively. Higher levels (up to 100%) of desorption were achieved by repeated elution or by an increase in surfactant concentration. Desorption of piscicolin with 1/10 volume of SDS solution resulted in a preparation with 10 times concentration in activity, equivalent to that of ammonium sulfate preparations (409,600 to 819,200 activity units/ml). Determination of organic nitrogen (N) content revealed that the desorbed piscicolin preparations were substantially free of proteinaceous substances (approximately 92 to 99%) compared with original culture supernatants and ammonium sulfate preparations. Nisin, pediocin, and brevicin were also desorbed with 1% SDS with a similar level of purification.
Authors:
M J Coventry; J B Gordon; M Alexander; M W Hickey; J Wan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-03     Completed Date:  1996-07-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1764-9     Citation Subset:  IM    
Affiliation:
Australian Food Industry Science Centre, Werribee, Victoria, Australia. coventryj@food.agvic.gov.au
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MeSH Terms
Descriptor/Qualifier:
Bacteriocins / isolation & purification*
Calcium Compounds / metabolism*
Food Microbiology*
Lactococcus / metabolism*
Silicates / metabolism*
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Calcium Compounds; 0/Silicates; 1344-95-2/calcium silicate
Comments/Corrections

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