| The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells. | |
| | |
MedLine Citation:
|
PMID: 22968515 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. METHOD: Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. RESULTS: S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the non-pregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. CONCLUSION: We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs. |
| | |
Authors:
|
Daniela Antunes Montani; Fernanda Bertuccez Cordeiro; Thaís Regiani; Amanda Begati Victorino; Eduardo Jorge Pilau; Fábio Cesar Gozzo; Christina Ramires Ferreira; Renato Fraietta; Edson Guimarães Lo Turco |
Related Documents
:
|
15818435 - Hyperemesis gravidarum presenting as jaundice and transient hyperthyroidism complicated... 18342435 - An autopsy case of acute multiple sclerosis (marburg's type) during pregnancy. 22093015 - Caring for women with unintended pregnancies. 22004905 - Regulatory t cells protect from autoimmune arthritis during pregnancy. 8030735 - Plasminogen activator of urokinase type and its inhibitor of placental type in hyperten... 7279345 - Histiocytosis x and pregnancy. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-12 |
Journal Detail:
|
Title: Journal of assisted reproduction and genetics Volume: - ISSN: 1573-7330 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-9-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Sao Paulo, SP, Brazil, Rua Embaú, 231, CEP: 04039-060. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sperm head vacuolization affects clinical outcome in ICSI cycle. A proposal of a cut-off value.
Next Document: CaCl(2) as a bifunctional reusable catalyst: diversity-oriented synthesis of 4H-pyran library under ...