Document Detail

The folate in human milk.
MedLine Citation:
PMID:  6896957     Owner:  NLM     Status:  MEDLINE    
In previous studies of the folate content of human milk, samples were prepared for assay by a method that resulted in a turbid solution that was then assayed by a turbidimetric microbiological method. We have used an improved microbiological assay in which the milks were treated with rennin to precipitate casein and heated in a buffered ascorbate to coagulate lactalbumin and lactoglobulin. Milks were obtained serially from nursing mothers for periods ranging from 1 day to 6 months postpartum. The results showed that the folate in human milk has few glutamate residues since treatment with a purified folate conjugase preparation release no additional folate activity for Lactobacillus casei. Colostrum is relatively low in folate, but milk folate increases as lactation proceeds. During each stage of lactation there was great variation in milk folate content among the women. In the case of a folate-deficient woman, supplementation with folic acid resulted in a prompt increase in milk folate level.
J M Cooperman; H S Dweck; L J Newman; C Garbarino; R Lopez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  36     ISSN:  0002-9165     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  576-80     Citation Subset:  AIM; IM    
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MeSH Terms
Colostrum / analysis
Folic Acid / analysis*
Milk, Human / analysis*
Nephelometry and Turbidimetry
gamma-Glutamyl Hydrolase / metabolism
Reg. No./Substance:
59-30-3/Folic Acid; EC Hydrolase

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