Document Detail


A focused microarray for screening rat embryonic stem cell lines.
MedLine Citation:
PMID:  22889370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here, we describe a focused microarray for screening rat embryonic stem cells (ESCs) and provide validation data that this array can distinguish undifferentiated rat ESCs from rat trophoblast stem (TS) cells, rat extraembryonic endoderm cells, mouse embryonic fibroblast feeder cells, and differentiated rat ESCs. Using this tool, genuine rat ESC lines, which have been expanded in a conventional rat ESC medium containing two inhibitors (2i), for example, glycogen synthase kinase 3 (GSK3) and mitogen-activated protein kinase (MEK) inhibitors, and leukemia inhibitory factor, and genuine rat ESCs, which have been expanded in rat ESC medium containing four inhibitors (4i), for example, GSK3, MEK, Alk5, and Rho-associated kinase inhibitors were compared; as were genuine rat ESCs from 4 different strains of rats. Expression of Cdx2, a gene associated with trophoblast determination, was observed in genuine, undifferentiated rat ESCs from 4 strains and from both 2i and 4i ESC derivation medium. This finding is in contrast to undifferentiated mouse ESCs that do not express Cdx2. The rat ESC focused microarray described in this report has utility for rapid screening of rat ESCs. This tool will enable optimization of culture conditions in the future.
Authors:
James Hong; Hong He; Phuoc Bui; Ben Ryba-White; Mohammad A K Rumi; Michael J Soares; Debasree Dutta; Soumen Paul; Masaki Kawamata; Takahiro Ochiya; Qi-Long Ying; Pavan Rajanahalli; Mark L Weiss
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-28
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-21     Completed Date:  2013-06-27     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  431-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Differentiation
Cell Line
Embryonic Stem Cells / metabolism*
Feeder Cells
Fibroblasts / metabolism
Genes, Essential
Germ Layers / cytology
Mice
Oligonucleotide Array Sequence Analysis*
Rats
Rats, Inbred F344
Rats, Long-Evans
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome*
Trophoblasts / cytology
Grant Support
ID/Acronym/Agency:
HD020676/HD/NICHD NIH HHS; NS34160/NS/NINDS NIH HHS; P20-RR017686/RR/NCRR NIH HHS; R01 OD010926/OD/NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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