| A fluorogenic, nucleic acid directed "click" reaction. | |
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MedLine Citation:
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PMID: 19769395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorogenic, nucleic acid directed, Cu(+)-catalyzed chemical reaction was developed that allows sequence-specific detection of nucleic acids at concentrations as low as 20 nM through monitoring of the increase of the fluorescence intensity. A single nucleotide mismatch in the template strand leads to the complete inhibition of this reaction. We observed that a Cu(+) ion stabilized with a water-soluble ligand is a significantly more efficient catalyst than free Cu(+). |
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Authors:
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Elmar Jentzsch; Andriy Mokhir |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Inorganic chemistry Volume: 48 ISSN: 1520-510X ISO Abbreviation: Inorg Chem Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-12 Completed Date: 2010-01-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0366543 Medline TA: Inorg Chem Country: United States |
Other Details:
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Languages: eng Pagination: 9593-5 Citation Subset: IM |
Affiliation:
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Anorganisch-Chemisches Institut, Ruprecht-Karls-Universit??t Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biosensing Techniques / methods* Catalysis Copper / chemistry* Fluorescent Dyes / chemical synthesis, chemistry* Limit of Detection Nucleic Acids / analysis* Spectrometry, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nucleic Acids; 7440-50-8/Copper |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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