Document Detail


A fluorescent redox dye. Influence of several substrates and electron carriers on the tetrazolium salt-formazan reaction of Ehrlich ascites tumour cells.
MedLine Citation:
PMID:  3583812     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was performed to elaborate the best conditions for measuring the redox activity of Ehrlich ascites tumour cells by using a new tetrazolium salt, cyantolyl tetrazolium chloride (CTC). This tetrazolium salt forms a fluorescent water-insoluble formazan on reduction on the surface of intact vital cells. The influences of fixation and of various substrates and electron carriers on the cellular reduction of CTC were investigated quantitatively using an elution technique. The amount of formazan obtained after incubating vital cells with Meldola Blue as electron carrier was greater than that obtained with Methylene Blue, menadione, 2,6-dichloroindophenol, 1-methoxyphenazine methosulphate or phenazine methosulphate. Using flow cytometry, the formazan production per cell and, after staining the nuclear DNA, the distribution of the redox activity in the cell population can be visualized with satisfactory resolution. We conclude from our findings that dehydrogenases are only partially involved in the reduction of tetrazolium salts by intact cells and that a redox activity, probably related to a cell membrane-bound NAD(P)H-oxidase system, is mainly measured.
Authors:
J Stellmach; E Severin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Histochemical journal     Volume:  19     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-07-10     Completed Date:  1987-07-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Ehrlich Tumor / metabolism*
Cyanides / metabolism
Electron Transport
Fluorescent Dyes
Formazans / metabolism
Mice
Oxidation-Reduction
Spectrometry, Fluorescence
Tetrazolium Salts* / metabolism
Chemical
Reg. No./Substance:
0/Cyanides; 0/Fluorescent Dyes; 0/Formazans; 0/Tetrazolium Salts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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