Document Detail


A fluorescence sensor for detection of geranyl pyrophosphate by the chemo-ensemble method.
MedLine Citation:
PMID:  19049370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hexaamide receptor 4 and coumarin phosphate 5 form an ensemble for effective sensing of geranyl pyrophosphate (GPP). Fluorescence resonance energy transfer in the 4 + 5 ensemble diminishes when 5 is replaced by GPP. Receptor 4 binds selectively with GPP over other anions, including fatty acids. The 4-GPP complex in 1:1 stoichiometry is inferred by NMR and fluorescence titrations. Receptor 4 contains a pseudotetrahedral cleft to accommodate GPP via multiple hydrogen bonds, and the two aliphatic chains exert additional hydrophobic interactions.
Authors:
Kuan-Hung Chen; Jen-Hai Liao; Hsin-Yu Chan; Jim-Min Fang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of organic chemistry     Volume:  74     ISSN:  1520-6904     ISO Abbreviation:  J. Org. Chem.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-09     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985193R     Medline TA:  J Org Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  895-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Amides / chemistry
Calibration
Coumarins / chemistry
Fluorescence
Fluorescent Dyes / chemistry*
Magnetic Resonance Spectroscopy
Polyisoprenyl Phosphates / analysis*
Titrimetry
Chemical
Reg. No./Substance:
0/Amides; 0/Coumarins; 0/Fluorescent Dyes; 0/Polyisoprenyl Phosphates; 763-10-0/geranyl pyrophosphate; 91-64-5/coumarin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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