| The fluorescence enhancement of quercetin-nucleic acid system and the analytical application. | |
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MedLine Citation:
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PMID: 19424980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for fsDNA, 5.0 x 10(-9) to 7.0 x 10(-7) g/mL for ctDNA and 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 x 10(-9), 7.8 x 10(-10) and 2.6 x 10(-9) g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system. |
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Authors:
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Yuan Liu; Xia Wu; Haiping Zhou; Xiaoyu Liu; Fengju Zhang; Jinghe Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Luminescence : the journal of biological and chemical luminescence Volume: 24 ISSN: 1522-7243 ISO Abbreviation: Luminescence Publication Date: 2009 Nov-Dec |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100889025 Medline TA: Luminescence Country: England |
Other Details:
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Languages: eng Pagination: 416-21 Citation Subset: IM |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes
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chemistry* Limit of Detection Molecular Structure Nucleic Acids / analysis*, chemistry Quercetin / chemistry* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nucleic Acids; 117-39-5/Quercetin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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