Document Detail


A fluorescence displacement assay for the measurement of arachidonoyl ethanolamide (anandamide) and oleoyl amide (octadecenoamide) hydrolysis.
MedLine Citation:
PMID:  9065749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a simple fluorescence displacement assay to measure hydrolysis of arachidonoyl ethanolamide and oleoyl amide, two important pharmacological compounds. Hydrolysis at the amide linkage of these ligands releases a fatty acid as one of the products. The displacement of a fluorescent fatty acid analogue from rat liver fatty acid-binding protein by the released fatty acid can thus be measured as a decrease in fluorescence. This process is time- and concentration-dependent and shows hyperbolic enzyme kinetics. Electrospray ionisation mass spectrometry was used to validate the assay.
Authors:
A E Thumser; J Voysey; D C Wilton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  53     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-04-07     Completed Date:  1997-04-07     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  433-5     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Southampton, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amides / metabolism
Animals
Arachidonic Acids / metabolism*
Cannabinoids / metabolism*
Carrier Proteins / metabolism
Fatty Acid-Binding Proteins
Fluorescence
Hydrolysis
Myelin P2 Protein / metabolism
Neoplasm Proteins*
Nerve Tissue Proteins*
Oleic Acids / metabolism
Polyunsaturated Alkamides
Rats
Sleep / drug effects
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Amides; 0/Arachidonic Acids; 0/Cannabinoids; 0/Carrier Proteins; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Myelin P2 Protein; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Oleic Acids; 0/Polyunsaturated Alkamides; 94421-68-8/anandamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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