| A fluorescence-based assay for quantitation of lymphokine-activated killer cell activity. | |
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MedLine Citation:
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PMID: 2785144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorescence assay for the quantitation of tumor cell lysis by activated and non-activated killer (LAK) cells is described. The target cells are labelled with a europium chelate (Eu-diethylenetriaminopentaacetate) and after cytolysis caused by the LAK cells the Eu3+ complex is released into the culture supernatant. The addition of beta-naphthoyltrifluoroacetone to culture supernatant aliquots leads to the formation of a highly fluorescent chelate which can be measured with a time-resolved fluorometer. The influence of various assay parameters has been evaluated including incubation time, effector-to-target cell ratio, the target cell line and different concentrations of interleukin-2 during cell culture. The optimized time-resolved fluorometric assay was found to be as simple and sensitive as the commonly used cytotoxicity assay in which the release of 51Cr from the labelled target cells is measured. In addition the assay is much faster and safer since the label is not radioactive. |
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Authors:
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T Volgmann; A Klein-Struckmeier; H Mohr |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunological methods Volume: 119 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1989 Apr |
Date Detail:
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Created Date: 1989-06-02 Completed Date: 1989-06-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 45-51 Citation Subset: IM |
Affiliation:
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Blood Transfusion Service of Lower Saxony, Department of Research and Development, Springe, F.R.G. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Communication Chelating Agents Chromium Radioisotopes / metabolism Cytotoxicity Tests, Immunologic* / methods Europium / metabolism Fluoroimmunoassay / methods* Humans Interleukin-2* Killer Cells, Natural / enzymology, immunology*, metabolism L-Lactate Dehydrogenase / metabolism Lymphocyte Activation* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Chromium Radioisotopes; 0/Interleukin-2; 7440-53-1/Europium; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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