| A fluorescence-based assay for monitoring clinical drug resistance. | |
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MedLine Citation:
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PMID: 22859393 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: Multidrug resistance (MDR) limits effectiveness in treating malignancy by modifying internalisation and/or externalisation of drugs through cancer cell membranes. In this study we describe an assay to monitor patients' responses to chemotherapy. METHODS: The assay is based on the fluorescent properties of doxorubicin alone as well as in combination with methotrexate, vinblastine, doxorubicin and cisplatin (MVAC). The slide-based cell imaging technique was first optimised using a panel of breast and urothelial cancer cell lines and then extended to fine needle breast aspiration biopsy and urine cytology. RESULTS: The drug fluorescence behaviour observed on smears of clinical specimens is identical to that obtained using fixed cultured cells. The fluorescence of sensitive cells to chemotherapy is mainly localised in the nucleus, whereas resistant cells show a weak fluorescence signal localised in the cytoplasm. The difference in terms of fluorescence intensity is also highlighted through fluorescence spectra. CONCLUSIONS: The results suggest that the assay provides clinically valuable information in predicting responses to doxorubicin and/or MVAC therapy. Originally set up on a confocal microscope, the assay was also effective using a standard epifluorescence microscope; as such it is technically simple, reliable and inexpensive. |
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Authors:
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Ariane Deniset-Besseau; François-Alexandre Miannay; Corinne Laplace-Builhé; Philippe Vielh; Sandrine Lécart; Bashir A Lwaleed; Pascal Eschwege; Marie-Pierre Fontaine-Aupart |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-2 |
Journal Detail:
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Title: Journal of clinical pathology Volume: - ISSN: 1472-4146 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institut des Sciences Moléculaires d'Orsay, Université Paris-Sud, Orsay, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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