| A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum. | |
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MedLine Citation:
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PMID: 20196166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. |
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Authors:
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Amy K Bei; Tiffany M Desimone; Aida S Badiane; Ambroise D Ahouidi; Tandakha Dieye; Daouda Ndiaye; Ousmane Sarr; Omar Ndir; Souleymane Mboup; Manoj T Duraisingh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of hematology Volume: 85 ISSN: 1096-8652 ISO Abbreviation: Am. J. Hematol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-31 Completed Date: 2010-04-19 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7610369 Medline TA: Am J Hematol Country: United States |
Other Details:
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Languages: eng Pagination: 234-7 Citation Subset: IM |
Affiliation:
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Harvard School of Public Health, Boston, Massachusetts, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Erythrocytes
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parasitology* Flow Cytometry / methods* Fluorescent Dyes Host-Parasite Interactions Humans Malaria, Falciparum / parasitology* Microscopy / methods Organic Chemicals Parasitemia / diagnosis Plasmodium falciparum / growth & development, pathogenicity*, physiology Sensitivity and Specificity Severity of Illness Index |
| Grant Support | |
ID/Acronym/Agency:
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1R03TW008053/TW/FIC NIH HHS; 5D43TW001503-09/TW/FIC NIH HHS; D43 TW001503-09/TW/FIC NIH HHS; R01 AI057919-05/AI/NIAID NIH HHS; R01AI057919/AI/NIAID NIH HHS; R03 TW008053-02/TW/FIC NIH HHS; R36 CK000119-01/CK/NCZVBED CDC HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Organic Chemicals; 163795-75-3/SYBR Green I |
| Comments/Corrections | |
Comment In:
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Am J Hematol. 2010 Apr;85(4):225-6
[PMID:
20201087
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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