| A flow cytometric method for determination of the blood neutrophil fraction in rats. | |
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MedLine Citation:
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PMID: 19383211 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Determination of the proportion of neutrophils in the peripheral blood is important for diagnostic purposes in medicine and for evaluating new drugs in the pharmaceutical industry. To measure the neutrophil concentration in rat blood, a fast and accurate flow cytometric method was developed. Rat neutrophils were quantified by using primary antibodies that recognize the RP1 antigen and secondary antibodies conjugated with fluorescein isothiocyanate. The flow cytometric method was calibrated by comparing cytometric results with data from a manual differential count. The results obtained by these 2 methods correlated with a Pearson correlation coefficient of 0.91 and were in agreement according to subsequent statistical analysis. To confirm the usefulness of the method in preclinical applications, the production of neutrophils in rats was stimulated by pegfilgrastim. Blood samples were taken at predetermined time points, and the pharmacodynamic profile was determined. These results confirmed that the flow cytometric method for neutrophil quantification is accurate and much faster than the manual microscopic method. Moreover, the flow cytometric method is easy to use, suggesting that it could become the method of choice for preclinical applications. |
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Authors:
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Spela Skrajnar; Marija Anzur Lasnik; Apolonija Bedina Zavec |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of the American Association for Laboratory Animal Science : JAALAS Volume: 48 ISSN: 1559-6109 ISO Abbreviation: J. Am. Assoc. Lab. Anim. Sci. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-22 Completed Date: 2009-05-15 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101269489 Medline TA: J Am Assoc Lab Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 152-6 Citation Subset: IM |
Affiliation:
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Lek Pharmaceuticals d d Ljubljana, Slovenia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation Female Flow Cytometry / methods* Laboratory Animal Science / methods* Leukocyte Count / methods* Male Microscopy / methods Neutrophils / cytology* Rats Rats, Wistar Reproducibility of Results |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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