| A flow cytometric assay for D8/17 B cell marker in patients with Tourette's syndrome and obsessive compulsive disorder. | |
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MedLine Citation:
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PMID: 9831399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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D8/17 is proving to be an important diagnostic and treatment tool in patients with Tourette's syndrome (TS) and obsessive compulsive disorder (OCD). The present study represents a direct comparison of immunofluorescence microscopy and flow cytometry in terms of their relative efficiency in the detection of the DB/17 B cell marker. Analysis of 41 patients with OCD or TS and 31 control subjects by immunofluorescence microscopy and flow cytometer demonstrated an average of 6.5% of D8/17-positive B cells in controls with an average of 19.6% in OCD patients. Using the flow cytometer, values of 6.2% for controls and 22.4% for OCD patients were obtained. The flow cytometer test was able to count many more cells, induce less operator error and offer faster analysis while correlating strongly with the microscopic technique. Overall in our sample, the FACS assay test has a sensitivity of 77% and a specificity of 87%. The ease of use and close correlation to disease activity makes this an ideal test for clinical laboratories. |
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Authors:
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F Chapman; K Visvanathan; R Carreño-Manjarrez; J B Zabriskie |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of immunological methods Volume: 219 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1998-12-07 Completed Date: 1998-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 181-6 Citation Subset: IM |
Affiliation:
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Laboratory of Clinical Microbiology and Immunology, The Rockefeller University, New York, NY 10021, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Analysis of Variance Animals Antibodies, Monoclonal / immunology* B-Lymphocytes / immunology* Biological Markers / blood Blood Child Flow Cytometry / methods* Humans Immunoglobulin M / immunology Isoantigens / blood*, immunology Mice Microscopy, Fluorescence / methods Obsessive-Compulsive Disorder / diagnosis* Sensitivity and Specificity Time Factors Tourette Syndrome / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/B-cell alloantigen D8-17; 0/Biological Markers; 0/Immunoglobulin M; 0/Isoantigens |
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