Document Detail


A flow cytometric assay for D8/17 B cell marker in patients with Tourette's syndrome and obsessive compulsive disorder.
MedLine Citation:
PMID:  9831399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D8/17 is proving to be an important diagnostic and treatment tool in patients with Tourette's syndrome (TS) and obsessive compulsive disorder (OCD). The present study represents a direct comparison of immunofluorescence microscopy and flow cytometry in terms of their relative efficiency in the detection of the DB/17 B cell marker. Analysis of 41 patients with OCD or TS and 31 control subjects by immunofluorescence microscopy and flow cytometer demonstrated an average of 6.5% of D8/17-positive B cells in controls with an average of 19.6% in OCD patients. Using the flow cytometer, values of 6.2% for controls and 22.4% for OCD patients were obtained. The flow cytometer test was able to count many more cells, induce less operator error and offer faster analysis while correlating strongly with the microscopic technique. Overall in our sample, the FACS assay test has a sensitivity of 77% and a specificity of 87%. The ease of use and close correlation to disease activity makes this an ideal test for clinical laboratories.
Authors:
F Chapman; K Visvanathan; R Carreño-Manjarrez; J B Zabriskie
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  219     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-07     Completed Date:  1998-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  181-6     Citation Subset:  IM    
Affiliation:
Laboratory of Clinical Microbiology and Immunology, The Rockefeller University, New York, NY 10021, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Animals
Antibodies, Monoclonal / immunology*
B-Lymphocytes / immunology*
Biological Markers / blood
Blood
Child
Flow Cytometry / methods*
Humans
Immunoglobulin M / immunology
Isoantigens / blood*,  immunology
Mice
Microscopy, Fluorescence / methods
Obsessive-Compulsive Disorder / diagnosis*
Sensitivity and Specificity
Time Factors
Tourette Syndrome / diagnosis*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/B-cell alloantigen D8-17; 0/Biological Markers; 0/Immunoglobulin M; 0/Isoantigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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