| A flexible protocol for cryopreservation of pronuclear and cleavage stage embryos created by conventional in vitro fertilization and intracytoplasmic sperm injection. | |
| | |
MedLine Citation:
|
PMID: 15541856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: To evaluate the hospital's cryopreservation protocol. STUDY DESIGN: A retrospective cohort analysis of 30 conventional IVF and 44 ICSI cycles in an assisted conception unit at a tertiary referral hospital. All supernumerary embryos were cryopreserved at the pronuclear or blastomere stage. The survival, morphology, implantation and pregnancy rates were evaluated. The chi2 test and Fisher's exact test were used to determine the statistical significance. RESULTS: A total of 327 pronuclear and cleavage stage embryos were cryopreserved. The post thaw survival rates of 107 conventional IVF and 220 ICSI embryos were 90.6% and 69.0%, respectively. Of the thawed cleavage stage embryos from 43 IVF and 88 ICSI cycles, 90.6% and 69.3% were intact, respectively. Of the thawed pronuclear stage embryos from 64 IVF and 132 ICSI cycles, 90.6% and 68.9% were intact with cleavage rates of 57.8% and 56%, respectively. In the final 27 conventional IVF and 41 ICSI frozen-thawed embryo transfer cycles, the pregnancy rates were 18.5% and 19.5%, respectively. CONCLUSIONS: The adopted cryopreservation protocol flexibly allows for the selection of cleavage-stage embryos for fresh embryo transfer and the cryopreservation of all supernumerary embryos at the pronuclear or the cleavage stage in a single cycle with satisfactory pregnancy rates. Further validation of this protocol is required. |
| | |
Authors:
|
Zouhair O Amarin |
Related Documents
:
|
8224276 - Influence of sperm parameters on embryo quality. 17118586 - Synchronization and superovulation of mature cycling gilts for the collection of pronuc... 16728196 - Field trial to compare pregnancy rates of bovine embryo cryopreservation methods: vitri... 8241436 - Cryopreservation of embryos and oocytes: an update. 10356176 - Role of hormonal contraceptives in prevention of pregnancy and sexually transmitted dis... 1778806 - Variance and covariance estimates for weaning weight of senepol cattle. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of obstetrics, gynecology, and reproductive biology Volume: 117 ISSN: 0301-2115 ISO Abbreviation: Eur. J. Obstet. Gynecol. Reprod. Biol. Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-11-15 Completed Date: 2005-04-08 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375672 Medline TA: Eur J Obstet Gynecol Reprod Biol Country: Ireland |
Other Details:
|
Languages: eng Pagination: 189-93 Citation Subset: IM |
Affiliation:
|
Department of Obstetrics and Gynaecology, Jordan University of Science and Technology, Irbid, P.O. Box 1572, Amman 11953, Jordan. zoamarin@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cleavage Stage, Ovum Clinical Protocols Cohort Studies Cryopreservation / methods* Embryo, Mammalian Female Fertilization in Vitro / methods* Humans Pregnancy Pregnancy Outcome Retrospective Studies Sperm Injections, Intracytoplasmic / methods Treatment Outcome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary.
Next Document: Embryo transfer is equally effective at cleavage stage and blastocyst stage: a randomized prospectiv...