Document Detail

A fission yeast gene for mitochondrial sulfide oxidation.
MedLine Citation:
PMID:  10224084     Owner:  NLM     Status:  MEDLINE    
A cadmium-hypersensitive mutant of the fission yeast Schizosaccharomyces pombe was found to accumulate abnormally high levels of sulfide. The gene required for normal regulation of sulfide levels, hmt2(+), was cloned by complementation of the cadmium-hypersensitive phenotype of the mutant. Cell fractionation and immunocytochemistry indicated that HMT2 protein is localized to mitochondria. Sequence analysis revealed homology between HMT2 and sulfide dehydrogenases from photosynthetic bacteria. HMT2 protein, produced in and purified from Escherichia coli, was soluble, bound FAD, and catalyzed the reduction of quinone (coenzyme Q2) by sulfide. HMT2 activity was also detected in isolated fission yeast mitochondria. We propose that HMT2 functions as a sulfide:quinone oxidoreductase. Homologous enzymes may be widespread in higher organisms, as sulfide-oxidizing activities have been described previously in animal mitochondria, and genes of unknown function, but with similarity to hmt2(+), are present in the genomes of flies, worms, rats, mice, and humans.
J G Vande Weghe; D W Ow
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-06-03     Completed Date:  1999-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13250-7     Citation Subset:  IM    
Plant Gene Expression Center, U. S. Department of Agriculture-Agricultural Research Service, Albany, California 94710, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF042283;  AF042284
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MeSH Terms
Amino Acid Sequence
Archaeal Proteins*
Bacterial Proteins / genetics*,  isolation & purification,  metabolism
Cloning, Molecular
DNA, Complementary
DNA-Binding Proteins / genetics*,  isolation & purification,  metabolism
Genes, Fungal*
Genetic Complementation Test
Mitochondria / metabolism*
Molecular Sequence Data
Schizosaccharomyces / genetics*,  metabolism
Sequence Homology, Amino Acid
Sulfides / metabolism*
Reg. No./Substance:
0/Archaeal Proteins; 0/Bacterial Proteins; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Sulfides; 149147-63-7/HmtB protein, Methanothermobacter thermautotrophicus

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