| The first and third intracellular loops together with the carboxy terminal tail of the delta-opioid receptor contribute toward functional interaction with Galpha16. | |
| | |
MedLine Citation:
|
PMID: 14535952 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Opioid peptides exert their regulatory effects on both central and peripheral nervous systems via multiple opioid receptors that are linked to seemingly identical sets of guanine nucleotide-binding regulatory proteins (G proteins). In contrast to the mu-opioid receptor, the delta-opioid receptor can efficiently stimulate phospholipase C via G16. We used a series of mu/delta-opioid receptor chimeras to examine the involvement of intracellular receptor domains in the recognition of G16. After ascertaining that the chimeras can bind opioid ligands with high affinity and elicit inhibition of adenylyl cyclase, COS-7 cells were cotransfected with cDNAs encoding Galpha16 and a mu/delta-opioid receptor chimera and assayed for [D-Ala2,D-Leu5]enkephalin-induced stimulation of phospholipase C. Our results indicate that (i) the carboxy terminal tail of the delta-opioid receptor is necessary but insufficient for conferring coupling to Galpha16, (ii) the third inner loop together with the carboxy terminal tail of the delta-opioid receptor can provide sufficient contact domains for Galpha16, and (iii) the first inner loop of the delta-opioid receptor, in particular Leu80, as well as the fifth transmembrane domain and/or the third extracellular loop may also contribute in defining the fidelity of interaction between the delta-opioid receptor and Galpha16. These results indicate that efficient coupling of the delta-opioid receptor to Galpha16 requires the participation of most of the intracellular regions, including the first intracellular loop. |
| | |
Authors:
|
Anthony S L Chan; Ping Y Law; Horace H Loh; Peter N N Ho; Wai-Man Wu; Joy S C Chan; Yung H Wong |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 87 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 2003 Nov |
Date Detail:
|
Created Date: 2003-10-10 Completed Date: 2003-11-24 Revised Date: 2012-07-12 |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
|
Languages: eng Pagination: 697-708 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals COS Cells Enkephalin, Leucine-2-Alanine / pharmacology GTP-Binding Protein alpha Subunits, Gq-G11 Heterotrimeric GTP-Binding Proteins / genetics, metabolism* Mice Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding / genetics, physiology Protein Structure, Tertiary / physiology Rats Receptors, Opioid, delta / genetics, metabolism* Receptors, Opioid, mu / genetics, metabolism Recombinant Fusion Proteins / genetics, metabolism Sequence Homology, Amino Acid Structure-Activity Relationship Transfection Type C Phospholipases / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, Opioid, delta; 0/Receptors, Opioid, mu; 0/Recombinant Fusion Proteins; 63631-40-3/Enkephalin, Leucine-2-Alanine; EC 3.1.4.-/Type C Phospholipases; EC 3.6.5.1/G protein alpha 16; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gq-G11; EC 3.6.5.1/Heterotrimeric GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Haloperidol impairs auditory filial imprinting and modulates monoaminergic neurotransmission in an i...
Next Document: Expression of BDNF mRNA in substantia nigra is dependent on target integrity and independent of neur...