Document Detail


A first-generation porcine whole-genome radiation hybrid map.
MedLine Citation:
PMID:  10430669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod >/= 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score >/=4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of approximately 70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.
Authors:
R J Hawken; J Murtaugh; G H Flickinger; M Yerle; A Robic; D Milan; J Gellin; C W Beattie; L B Schook; L J Alexander
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mammalian genome : official journal of the International Mammalian Genome Society     Volume:  10     ISSN:  0938-8990     ISO Abbreviation:  Mamm. Genome     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-02     Completed Date:  1999-09-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100916     Medline TA:  Mamm Genome     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  824-30     Citation Subset:  IM    
Affiliation:
Program on Comparative Genomics, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, Minnesota 55108, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Mapping / methods,  veterinary*
Chromosomes, Artificial, Yeast / genetics
Genetic Markers
Genome*
Hybrid Cells
Linkage (Genetics)
Polymerase Chain Reaction
Swine / genetics*
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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