Document Detail


The first cell cycle after transfer of somatic cell nuclei in a non-human primate.
MedLine Citation:
PMID:  15128675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Production of genetically identical non-human primates through somatic cell nuclear transfer (SCNT) can provide diseased genotypes for research and clarify embryonic stem cell potentials. Understanding the cellular and molecular changes in SCNT is crucial to its success. Thus the changes in the first cell cycle of reconstructed zygotes after nuclear transfer (NT) of somatic cells in the Long-tailed Macaque (Macaca fascicularis) were studied. Embryos were reconstructed by injecting cumulus and fibroblasts from M. fascicularis and M. silenus, into enucleated M. fascicularis oocytes. A spindle of unduplicated premature condensed chromosome (PCC spindle) from the donor somatic cell was formed at 2 hours after NT. Following activation, the chromosomes segregated and moved towards the two PCC spindle poles, then formed two nuclei. Twenty-four hours after activation, the first cell division occurred. A schematic of the first cell cycle changes following injection of a somatic cell into an enucleated oocyte is proposed. Ninety-three reconstructed embryos were transferred into 31 recipients, resulting in 7 pregnancies that were confirmed by ultrasound; unfortunately none progressed beyond 60 days.
Authors:
Soon-Chye Ng; Naiqing Chen; Wan-Yue Yip; Swee-Lian Liow; Guo-Qing Tong; Barbara Martelli; Lay Geok Tan; Paolo Martelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  131     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-06     Completed Date:  2004-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2475-84     Citation Subset:  IM    
Affiliation:
Laboratory of Nuclear Reprogramming and Cell Differentiation, Department of Obstetrics and Gynaecology, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore. scng@embryonics.biz
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / physiology*
Cell Division
Cell Nucleus / physiology,  ultrastructure*
Cells, Cultured
Embryonic and Fetal Development
Female
Fibroblasts / cytology*
Flow Cytometry
Genotype
Macaca fascicularis / embryology*
Male
Nuclear Transfer Techniques*
Pregnancy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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